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ADIPOQ ELISA 试剂盒

Name: ADIPOQ ELISA 试剂盒
SKU: ABIN411385
Availability: OutOfStock

This 小鼠 ADIPOQ ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 ADIPOQ in Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate 和 Urine. This ELISA Kit has been cited in 13+ publications.

ELISA

1 image

(13 references)

产品编号 ABIN411385

发货至: 中国

Datasheet as PDF

Quick Overview for ADIPOQ ELISA 试剂盒 (ABIN411385)

抗原

See all ADIPOQ ELISA试剂盒

ADIPOQ (Adiponectin (ADIPOQ))

抗原表位

AA 18-247

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

312-20000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate, Urine

Discover our top product ADIPOQ ELISA Kit

最低检测浓度

312 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Adiponectin

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: NSO
Immunogen sequence: E18-N247

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<10pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: NSO
Immunogen sequence: E18-N247
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Adiponectin (ADIPOQ) ELISA Kit
ADIPOQ 适用: 马 Colorimetric Sandwich ELISA 0.156 μg/mL - 10 μg/mL Plasma, Serum, Tissue Homogenate

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Sequence similarities: Contains 1 C1q domain.
Tissue Specificity: Synthesized exclusively by adipocytes and secreted into plasma.

板类型

Pre-coated

实验流程

mouse Adiponectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Adiponectin has been precoated onto 96-well plates. Standards(NSO, E18-N247) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Adiponectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Adiponectin amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse Adiponectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Adiponectin standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(ng/ml): 3.35, Standard deviation: 0.160, CV(%): 5
- Sample 2: n=16, Mean(ng/ml): 6.82, Standard deviation: 0.360, CV(%): 5.3
- Sample 3: n=16, Mean(ng/ml): 13.50, Standard deviation: 0.756, CV(%): 5.6,
- Sample 1: n=24, Mean(ng/ml): 3.39, Standard deviation: 0.190, CV(%): 5.4
- Sample 2: n=24, Mean(ng/ml): 6.60, Standard deviation: 0.380, CV(%): 5.8
- Sample 3: n=24, Mean(ng/ml): 13.20, Standard deviation: 0.818, CV(%): 6.2
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Chen, Wang, Hu, Zhou, Xu, Qiu, Qin, Zhang, Ying: "Programming of mouse obesity by maternal exposure to concentrated ambient fine particles." in: Particle and fibre toxicology, Vol. 14, Issue 1, pp. 20, (2017) (PubMed).

Jiang, Li, Yu, Liu, Xu, He, Gu, Liu: "Oral Administration of Resveratrol Alleviates Osteoarthritis Pathology in C57BL/6J Mice Model Induced by a High-Fat Diet." in: Mediators of inflammation, Vol. 2017, pp. 7659023, (2017) (PubMed).

Xu, Li, Dong, Tan, Wu, Xie, Yang: "Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE-/- Mice through the ROS/MAPK/NF-κB Pathway." in: Nutrients, Vol. 7, Issue 8, pp. 7085-105, (2016) (PubMed).

Yang, Yao, Yang, Wei, Peng, Ding, Shu: "Puerarin Protects Pancreatic β-Cells in Obese Diabetic Mice via Activation of GLP-1R Signaling." in: Molecular endocrinology (Baltimore, Md.), Vol. 30, Issue 3, pp. 361-71, (2016) (PubMed).

Wu, Chen: "Extracted Triterpenes from Antrodia cinnamomea Reduce the Inflammation to Promote the Wound Healing via the STZ Inducing Hyperglycemia-Diabetes Mice Model." in: Frontiers in pharmacology, Vol. 7, pp. 154, (2016) (PubMed).

Rasekhi, Karandish, Jalali, Mohammadshahi, Zarei, Saki, Shahbazian: "Phylloquinone supplementation improves glycemic status independent of the effects of adiponectin levels in premonopause women with prediabetes: a double-blind randomized controlled clinical trial." in: Journal of diabetes and metabolic disorders, Vol. 14, Issue 1, pp. 1, (2015) (PubMed).

Wu, Weng, Lai, Tsai, Liu, Lee, Tsai: "Effect of Lactobacillus plantarum Strain K21 on High-Fat Diet-Fed Obese Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 391767, (2015) (PubMed).

Qin, Ma, Yang, Hu, Zhou, Fu, Tian, Liu, Xu, Shen: "A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes." in: Natural products and bioprospecting, Vol. 5, Issue 3, pp. 159-66, (2015) (PubMed).

Liu, Gu, Zhao, An, Yang: "Glypican 4 may be involved in the adipose tissue redistribution in high-fat feeding C57BL/6J mice with peroxisome proliferators-activated receptor ? agonist rosiglitazone treatment." in: Experimental and therapeutic medicine, Vol. 8, Issue 6, pp. 1813-1818, (2014) (PubMed).

Liu, Liu, Li, Han, Peng, Shen: "Adiponectin is expressed in the pancreas of high-fat-diet-fed mice and protects pancreatic endothelial function during the development of type 2 diabetes." in: Diabetes & metabolism, Vol. 40, Issue 5, pp. 363-72, (2014) (PubMed).

Hatami Zargaran, Salehi, Heydari, Babajafari: "The Effects of 6 Isocaloric Meals on Body Weight, Lipid Profiles, Leptin, and Adiponectin in Overweight Subjects (BMI > 25)." in: International cardiovascular research journal, Vol. 8, Issue 2, pp. 52-6, (2014) (PubMed).

Liu, Xue, Liu, Lou, Wang, Yanagita, Xue, Wang: "Eicosapentaenoic acid-enriched phospholipid ameliorates insulin resistance and lipid metabolism in diet-induced-obese mice." in: Lipids in health and disease, Vol. 12, pp. 109, (2013) (PubMed).

Chen, Li, Zhang, Li, Sun, Zhang, Feng, Zhu: "Effects of resveratrol on the amelioration of insulin resistance in KKAy mice." in: Canadian journal of physiology and pharmacology, Vol. 90, Issue 2, pp. 237-42, (2012) (PubMed).
抗原 See all ADIPOQ ELISA试剂盒

ADIPOQ (Adiponectin (ADIPOQ))

别名

ADIPOQ

背景

Protein Function: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. .
Background: Adiponectin(ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.
Synonyms: Adiponectin,30 kDa adipocyte complement-related protein,Adipocyte complement-related 30 kDa protein,ACRP30,Adipocyte, C1q and collagen domain-containing protein,Adipocyte-specific protein AdipoQ,Adipoq,Acdc, Acrp30, Apm1,
Full Gene Name: Adiponectin
Cellular Localisation: Secreted.

基因ID

11450

UniProt

Q60994