ADIPOQ ELISA 试剂盒
(12 references)Quick Overview for ADIPOQ ELISA 试剂盒 (ABIN411384)
抗原
See all ADIPOQ ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.56 ng/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Adiponectin
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: E19-N244 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <60pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: E19-N244
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Adiponectin (ADIPOQ) ELISA Kit
ADIPOQ 适用: 人 Colorimetric Sandwich ELISA 1.562-100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 绵羊 Colorimetric Competition ELISA 6.25 ng/mL - 100 ng/mL Plasma, Serum
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 小鸡 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 小鼠 AA 18-247 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate, Urine
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 马 Colorimetric Sandwich ELISA 0.156 μg/mL - 10 μg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- human Adiponectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Adiponectin has been precoated onto 96-well plates. Standards(NSO, E19-N244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Adiponectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Adiponectin amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human Adiponectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Adiponectin standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 13.8, Standard deviation: 0.39, CV(%): 2.8
- Sample 2: n=16, Mean(ng/ml): 37.2, Standard deviation: 1.34, CV(%): 3.6
- Sample 3: n=16, Mean(ng/ml): 67.3, Standard deviation: 3.03, CV(%): 4.5,
- Sample 1: n=24, Mean(ng/ml): 13.1, Standard deviation: 0.77, CV(%): 5.9
- Sample 2: n=24, Mean(ng/ml): 36.8, Standard deviation: 2.50, CV(%): 6.8
- Sample 3: n=24, Mean(ng/ml): 65.7, Standard deviation: 4.66, CV(%): 7.1
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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: "Elective percutaneous coronary intervention leads to significant changes in serum resistin, leptin, and adiponectin levels regardless of periprocedural myocardial injury: an observational study." in: Anatolian journal of cardiology, Vol. 16, Issue 12, pp. 940-946, (2018) (PubMed).
: "Determining residual adipose tissue characteristics with MRI in patients with various subtypes of lipodystrophy." in: Diagnostic and interventional radiology (Ankara, Turkey), Vol. 23, Issue 6, pp. 428-434, (2018) (PubMed).
: "The Relationship between Serum Zonulin Level and Clinical and Laboratory Parameters of Childhood Obesity." in: Journal of clinical research in pediatric endocrinology, Vol. 9, Issue 1, pp. 31-38, (2017) (PubMed).
: "Responses of inflammatory cytokines following moderate intensity walking exercise in overweight or obese individuals." in: Journal of exercise rehabilitation, Vol. 13, Issue 4, pp. 472-476, (2017) (PubMed).
: "Adiponectin Concentration in Gestational Diabetic Women: a Case-Control Study." in: Clinical nutrition research, Vol. 6, Issue 4, pp. 267-276, (2017) (PubMed).
: "The effect of vitamin D administration on serum leptin and adiponectin levels in end-stage renal disease patients on hemodialysis with vitamin D deficiency: A placebo-controlled double-blind clinical ..." in: Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, Vol. 21, pp. 1, (2016) (PubMed).
: "Effect of Lactobacillus plantarum Strain K21 on High-Fat Diet-Fed Obese Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 391767, (2015) (PubMed).
: "Phylloquinone supplementation improves glycemic status independent of the effects of adiponectin levels in premonopause women with prediabetes: a double-blind randomized controlled clinical trial." in: Journal of diabetes and metabolic disorders, Vol. 14, Issue 1, pp. 1, (2015) (PubMed).
: "Adiponectin is expressed in the pancreas of high-fat-diet-fed mice and protects pancreatic endothelial function during the development of type 2 diabetes." in: Diabetes & metabolism, Vol. 40, Issue 5, pp. 363-72, (2014) (PubMed).
: "The Effects of 6 Isocaloric Meals on Body Weight, Lipid Profiles, Leptin, and Adiponectin in Overweight Subjects (BMI > 25)." in: International cardiovascular research journal, Vol. 8, Issue 2, pp. 52-6, (2014) (PubMed).
: "Glypican 4 may be involved in the adipose tissue redistribution in high-fat feeding C57BL/6J mice with peroxisome proliferators-activated receptor ? agonist rosiglitazone treatment." in: Experimental and therapeutic medicine, Vol. 8, Issue 6, pp. 1813-1818, (2014) (PubMed).
: "Effects of resveratrol on the amelioration of insulin resistance in KKAy mice." in: Canadian journal of physiology and pharmacology, Vol. 90, Issue 2, pp. 237-42, (2012) (PubMed).
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: "Elective percutaneous coronary intervention leads to significant changes in serum resistin, leptin, and adiponectin levels regardless of periprocedural myocardial injury: an observational study." in: Anatolian journal of cardiology, Vol. 16, Issue 12, pp. 940-946, (2018) (PubMed).
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- ADIPOQ (Adiponectin (ADIPOQ))
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别名
- ADIPOQ
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背景
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Background: Adiponectin(ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226(19-244) amino acids with the molecular mass of 36KDa after glycosylation.
Synonyms: Adiponectin ,Adiponectin, C1Q and collagen domain containing ,cDNA FLJ78108, highly similar to Homo sapiens adiponectin, C1Q and collagen domain containing (ADIPOQ), mRNA ,ADIPOQ ,hCG_1784052 ,
Full Gene Name: adiponectin, C1Q and collagen domain containing
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基因ID
- 9370
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UniProt
- A8K660
抗原 See all ADIPOQ ELISA试剂盒
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