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LTA ELISA 试剂盒

Name: LTA ELISA 试剂盒
SKU: ABIN411381
Availability: OutOfStock

This 化学剂 LTA ELISA Kit is a Colorimetric ELISA Kit designed to quantify 化学剂 LTA in Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA) 和 Plasma (citrate). This ELISA Kit has been cited in 2+ publications.

ELISA

1 image

(2 references)

产品编号 ABIN411381

发货至: 中国

Datasheet as PDF

Quick Overview for LTA ELISA 试剂盒 (ABIN411381)

抗原

See all LTA ELISA试剂盒

LTA (Lymphotoxin-alpha (LTA))

抗原表位

AA 35-205

适用

化学剂

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5-4000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Discover our top product LTA ELISA Kit

最低检测浓度

62.5 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNF beta

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: L35-L205

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<5pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: E.coli
Immunogen sequence: L35-L205
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LTA 适用: 化学剂 Colorimetric 7.8-500 pg/mL Plasma, Serum, Tissue Homogenate

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Sequence similarities: Belongs to the tumor necrosis factor family.

板类型

Pre-coated

实验流程

human TNF beta ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNF beta has been precoated onto 96-well plates. Standards(E.coli, L35-L205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNF beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNF beta amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TNF beta standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TNF beta standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 415, Standard deviation: 23.24, CV(%): 5.6
- Sample 2: n=16, Mean(pg/ml): 1952, Standard deviation: 68.32, CV(%): 3.5
- Sample 3: n=16, Mean(pg/ml): 2822, Standard deviation: 129.8, CV(%): 4.6,
- Sample 1: n=24, Mean(pg/ml): 660, Standard deviation: 40.92, CV(%): 6.2
- Sample 2: n=24, Mean(pg/ml): 2546, Standard deviation: 122.2, CV(%): 4.8
- Sample 3: n=24, Mean(pg/ml): 3148, Standard deviation: 210.9, CV(%): 6.7
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Wang, Zheng, Meng, Wang, He, Zhang, Liu, Hu, He, Hu, Wang: "In vivo study of immunogenicity and kinetic characteristics of a quantum dot-labelled baculovirus." in: Biomaterials, Vol. 64, pp. 78-87, (2015) (PubMed).

Zeng, Huang, Zheng, Guo, Pan: "Effects of reactive nitrogen scavengers on NK-cell-mediated killing of K562 cells." in: Journal of biomedicine & biotechnology, Vol. 2012, pp. 101737, (2012) (PubMed).
抗原 See all LTA ELISA试剂盒

LTA (Lymphotoxin-alpha (LTA))

别名

LTA

物质类

Chemical

背景

Protein Function: Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.
Background: Tumor necrosis factor-beta(TNF-beta), previously called lymphotoxin-alpha(LTA), is a cytokine produced by lymphocytes. TNF-alpha and TNF-beta share 35 % identity and 50 % homology in the amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immunostimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with the molecular mass of 18.8KDa.
Synonyms: Lymphotoxin-alpha,LT-alpha,TNF-beta,Tumor necrosis factor ligand superfamily member 1,LTA,TNFB, TNFSF1,
Full Gene Name: Lymphotoxin-alpha
Cellular Localisation: Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated.

基因ID

4049

UniProt

P01374

途径

Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process