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Resistin ELISA 试剂盒

Name: Resistin ELISA 试剂盒
SKU: ABIN411379
Availability: OutOfStock

This anti-Resistin antibody (ABIN411379) is a antibody detecting Resistin in ELISA. Suitable for Human.

ELISA

1 image

(2 references)

产品编号 ABIN411379

发货至: 中国

Datasheet as PDF

Quick Overview for Resistin ELISA 试剂盒 (ABIN411379)

抗原

See all Resistin (RETN) ELISA试剂盒

Resistin (RETN)

抗原表位

AA 19-108

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

78-5000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

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最低检测浓度

78 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Resistin

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: K19-P108

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<3pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: E.coli
Immunogen sequence: K19-P108
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应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Sequence similarities: Belongs to the resistin/FIZZ family.
Tissue Specificity: Expressed only in fatty tissues.

板类型

Pre-coated

实验流程

human Resistin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Resistin has been precoated onto 96-well plates. Standards(E.coli, K19-P108) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Resistin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Resistin amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human Resistin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin , EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Resistin standard solution and each sample is measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 534, Standard deviation: 28.84, CV(%): 5.4
- Sample 2: n=16, Mean(pg/ml): 1453, Standard deviation: 88.63, CV(%): 6.1
- Sample 3: n=16, Mean(pg/ml): 2323, Standard deviation: 109.2, CV(%): 4.7,
- Sample 1: n=24, Mean(pg/ml): 554, Standard deviation: 45.98, CV(%): 8.3
- Sample 2: n=24, Mean(pg/ml): 1532, Standard deviation: 119.5, CV(%): 7.8
- Sample 3: n=24, Mean(pg/ml): 2486, Standard deviation: 200, CV(%): 8
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Montazerifar, Bolouri, Paghalea, Mahani, Karajibani: "Obesity, Serum Resistin and Leptin Levels Linked to Coronary Artery Disease." in: Arquivos brasileiros de cardiologia, Vol. 107, Issue 4, pp. 348-353, (2017) (PubMed).

Niu, Li, Li, Song, Jin, Liu: "Serum resistin positively correlates with serum lipids, but not with insulin resistance, in first-degree relatives of type-2 diabetes patients: an observational study in China." in: Medicine, Vol. 96, Issue 16, pp. e6622, (2017) (PubMed).
抗原 See all Resistin (RETN) ELISA试剂盒

Resistin (RETN)

别名

RETN

背景

Protein Function: Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.
Background: Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance.1 Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin(for resistance to insulin), that may be the hormone potentially links obesity to diabetes.2 Resistin is overexpressed in human adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.3 The standard product used in this kit is human Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.
Synonyms: Resistin,Adipose tissue-specific secretory factor,ADSF,C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein,Cysteine-rich secreted protein A12-alpha-like 2,Cysteine-rich secreted protein FIZZ3,RETN,FIZZ3, HXCP1, RSTN,UNQ407/PRO1199,
Full Gene Name: Resistin
Cellular Localisation: Secreted.

基因ID

56729

UniProt

Q9HD89

途径

Feeding Behaviour, Smooth Muscle Cell Migration