TIMP3 ELISA 试剂盒
(1 reference)Quick Overview for TIMP3 ELISA 试剂盒 (ABIN411360)
抗原
See all TIMP3 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TIMP-3
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: C24-P211 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <2pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: C24-P211
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TIMP Metallopeptidase Inhibitor 3 (TIMP3) ELISA Kit
TIMP3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 3 (TIMP3) ELISA KitTIMP3 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 3 (TIMP3) ELISA KitTIMP3 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 3 (TIMP3) ELISA KitTIMP3 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
TIMP Metallopeptidase Inhibitor 3 (TIMP3) ELISA KitTIMP3 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 3 (TIMP3) ELISA KitTIMP3 适用: 小鸡 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 3 (TIMP3) ELISA KitTIMP3 适用: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 3 (TIMP3) ELISA KitTIMP3 适用: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 3 (TIMP3) ELISA KitTIMP3 适用: 马 Colorimetric Sandwich ELISA 0.47 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 3 (TIMP3) ELISA KitTIMP3 适用: 山羊 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family.
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板类型
- Pre-coated
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实验流程
- human TIMP-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIMP-3 has been precoated onto 96-well plates. Standards(NSO, C24-P211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TIMP-3 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TIMP-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin , EDTA) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TIMP-3 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 1250, Standard deviation: 72.5, CV(%): 5.8
- Sample 2: n=16, Mean(pg/ml): 2687, Standard deviation: 110.2, CV(%): 4.1
- Sample 3: n=16, Mean(pg/ml): 5353, Standard deviation: 192.7, CV(%): 3.6,
- Sample 1: n=24, Mean(pg/ml): 1430, Standard deviation: 95.81, CV(%): 6.7
- Sample 2: n=24, Mean(pg/ml): 2832, Standard deviation: 147.3, CV(%): 5.2
- Sample 3: n=24, Mean(pg/ml): 5657, Standard deviation: 277.2, CV(%): 4.9
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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: "Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases and Their Inhibitors in the Osteoarthritic Cartilage In Vitro." in: Stem cells international, Vol. 2017, pp. 9542702, (2017) (PubMed).
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: "Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases and Their Inhibitors in the Osteoarthritic Cartilage In Vitro." in: Stem cells international, Vol. 2017, pp. 9542702, (2017) (PubMed).
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- TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))
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别名
- TIMP3
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背景
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Protein Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
Background: The tissue inhibitors of metalloproteinases(TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy(SFD).
Synonyms: Metalloproteinase inhibitor 3,Protein MIG-5,Tissue inhibitor of metalloproteinases 3,TIMP-3,TIMP3,
Full Gene Name: Metalloproteinase inhibitor 3
Cellular Localisation: Secreted, extracellular space, extracellular matrix. -
基因ID
- 7078
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UniProt
- P35625
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