P-Selectin ELISA 试剂盒
(4 references)Quick Overview for P-Selectin ELISA 试剂盒 (ABIN411352)
抗原
See all P-Selectin (SELP) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse P-Selectin
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: W42-A709 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <5pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: W42-A709
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Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA Kit
SELP 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 兔 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: Pig Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 犬 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Plasma, Serum, Tissue Homogenate
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 兔 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Saliva, Serum, Tissue Homogenate, Urine
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 人 AA 42-771 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 人 Soluble Colorimetric Sandwich ELISA 78.13-5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 猴 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the selectin/LECAM family.
Tissue Specificity: Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
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板类型
- Pre-coated
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实验流程
- mouse P-Selectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for P-Selectin has been precoated onto 96-well plates. Standards(NSO, W42-A709) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse P-Selectin amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse P-Selectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse P-Selectin standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 325, Standard deviation: 14, CV(%): 4.3
- Sample 2: n=16, Mean(pg/ml): 874, Standard deviation: 43.7, CV(%): 5
- Sample 3: n=16, Mean(pg/ml): 1886, Standard deviation: 71.7, CV(%): 3.8,
- Sample 1: n=24, Mean(pg/ml): 417, Standard deviation: 27.11, CV(%): 6.5
- Sample 2: n=24, Mean(pg/ml): 1029, Standard deviation: 63.8, CV(%): 6.2
- Sample 3: n=24, Mean(pg/ml): 2020, Standard deviation: 94.94, CV(%): 4.7
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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: "Recruitment of HIV-1 target cells at topical mucosal sites: a sensitive and early marker for determining the safety of microbicide candidates." in: Emerging microbes & infections, Vol. 2, Issue 7, pp. e42, (2015) (PubMed).
: "Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis." in: Free radical biology & medicine, Vol. 74, pp. 108-17, (2014) (PubMed).
: "L-selectin and P-selectin are novel biomarkers of cervicovaginal inflammation for preclinical mucosal safety assessment of anti-HIV-1 microbicide." in: Antimicrobial agents and chemotherapy, Vol. 56, Issue 6, pp. 3121-32, (2012) (PubMed).
: "The detrimental effect of nitric oxide on tissue is associated with inflammatory events in the vascular endothelium and neutrophils in mice with dextran sodium sulfate-induced colitis." in: Free radical research, Vol. 46, Issue 12, pp. 1427-36, (2012) (PubMed).
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: "Recruitment of HIV-1 target cells at topical mucosal sites: a sensitive and early marker for determining the safety of microbicide candidates." in: Emerging microbes & infections, Vol. 2, Issue 7, pp. e42, (2015) (PubMed).
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- P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))
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别名
- SELP
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背景
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Protein Function: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. .
Background: P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1(PSGL-1). The standard product used in this kit is recombinant mouse P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 42-709 amino acids sequence with the molecular mass of 99.1KDa. As a result of glycosylation, the molecular mass of 190-200KDa is revealed by SDS-PAGE.
Synonyms: P-selectin,CD62 antigen-like family member P,Granule membrane protein 140,GMP-140,Leukocyte-endothelial cell adhesion molecule 3,LECAM3,Platelet activation dependent granule-external membrane protein,PADGEM,CD62P,Selp,Grmp,
Full Gene Name: P-selectin
Cellular Localisation: Membrane, Single-pass type I membrane protein. -
基因ID
- 20344
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UniProt
- Q01102
抗原 See all P-Selectin (SELP) ELISA试剂盒
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