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Leptin ELISA 试剂盒

LEP 适用: 人 AA 22-167 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
产品编号 ABIN411318
发货至: 中国
  • 抗原 See all Leptin (LEP) ELISA试剂盒
    Leptin (LEP)
    抗原表位
    AA 22-167
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    62.5-4000 pg/mL
    最低检测浓度
    62.5 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Leptin
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: E.coli
    Immunogen sequence: V22-C167
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <10pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: E.coli
    Immunogen sequence: V22-C167
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    板类型
    Pre-coated
    实验流程
    human Leptin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Leptin has been precoated onto 96-well plates. Standards(E.coli, V22-C167) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Leptin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Leptin amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Leptin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Leptin standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 1026, Standard deviation: 37.96, CV(%): 3.7
    • Sample 2: n=16, Mean(pg/ml): 2535, Standard deviation: 81.12, CV(%): 3.2
    • Sample 3: n=16, Mean(pg/ml): 3542, Standard deviation: 123.97, CV(%): 3.5,
    • Sample 1: n=24, Mean(pg/ml): 990, Standard deviation: 57.42, CV(%): 5.8
    • Sample 2: n=24, Mean(pg/ml): 2674, Standard deviation: 120.33, CV(%): 4.5
    • Sample 3: n=24, Mean(pg/ml): 3305, Standard deviation: 125.59, CV(%): 3.8
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • Buturak, Değirmencioğlu, Bayrak, Kırış, Karakurt, Demir, Sürgit, Ertürk: "Elective percutaneous coronary intervention leads to significant changes in serum resistin, leptin, and adiponectin levels regardless of periprocedural myocardial injury: an observational study." in: Anatolian journal of cardiology, Vol. 16, Issue 12, pp. 940-946, (2018) (PubMed).

    He, Le, Frediani, Winterberg, Jin, Liverman, Hernandez, Cleeton, Vos: "Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA." in: Pediatric transplantation, Vol. 21, Issue 6, (2018) (PubMed).

    Acar, Paketçi, Küme, Tuhan, Gürsoy Çalan, Demir, Böber, Abacı: "Serum galectin-1 levels are positively correlated with body fat and negatively with fasting glucose in obese children." in: Peptides, Vol. 95, pp. 51-56, (2018) (PubMed).

    Altay, Seçil, Demir, Atik, Akıncı, Özdemir Kutbay, Keskin Temeloğlu, Yıldırım Şimşir, Özışık, Demir, Eren, Tuna, Aytaç, Onay, Akıncı: "Determining residual adipose tissue characteristics with MRI in patients with various subtypes of lipodystrophy." in: Diagnostic and interventional radiology (Ankara, Turkey), Vol. 23, Issue 6, pp. 428-434, (2018) (PubMed).

    Algul, Ozdenk, Ozcelik: "Variations in leptin, nesfatin-1 and irisin levels induced by aerobic exercise in young trained and untrained male subjects." in: Biology of sport, Vol. 34, Issue 4, pp. 339-344, (2018) (PubMed).

    Küme, Acar, Tuhan, Çatlı, Anık, Gürsoy Çalan, Böber, Abacı: "The Relationship between Serum Zonulin Level and Clinical and Laboratory Parameters of Childhood Obesity." in: Journal of clinical research in pediatric endocrinology, Vol. 9, Issue 1, pp. 31-38, (2017) (PubMed).

    Tokgöz, Işık, Akbari, Kume, Sayın, Erdal, Arslan: "Perilipin polymorphisms are risk factors for the development of obesity in adolescents? A case-control study." in: Lipids in health and disease, Vol. 16, Issue 1, pp. 52, (2017) (PubMed).

    Naini, Vahdat, Hedaiati, Shahzeidi, Pezeshki, Nasri et al.: "The effect of vitamin D administration on serum leptin and adiponectin levels in end-stage renal disease patients on hemodialysis with vitamin D deficiency: A placebo-controlled double-blind clinical ..." in: Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, Vol. 21, pp. 1, (2016) (PubMed).

    Wu, Weng, Lai, Tsai, Liu, Lee, Tsai: "Effect of Lactobacillus plantarum Strain K21 on High-Fat Diet-Fed Obese Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 391767, (2015) (PubMed).

    Çatl?, An?k, Küme, Çalan, Dündar, Böber, Abac?: "Serum nesfatin-1 and leptin levels in non-obese girls with premature thelarche." in: Journal of endocrinological investigation, Vol. 38, Issue 8, pp. 909-13, (2015) (PubMed).

    Rasekhi, Karandish, Jalali, Mohammadshahi, Zarei, Saki, Shahbazian: "Phylloquinone supplementation improves glycemic status independent of the effects of adiponectin levels in premonopause women with prediabetes: a double-blind randomized controlled clinical trial." in: Journal of diabetes and metabolic disorders, Vol. 14, Issue 1, pp. 1, (2015) (PubMed).

    Yang, Zhang, Fu, Yang: "Leptin and IL-8: two novel cytokines screened out in childhood lead exposure." in: Toxicology letters, Vol. 227, Issue 3, pp. 172-8, (2014) (PubMed).

    Hajihashemi, Azadbakht, Hashemipor, Kelishadi, Esmaillzadeh: "Whole-grain intake favorably affects markers of systemic inflammation in obese children: a randomized controlled crossover clinical trial." in: Molecular nutrition & food research, Vol. 58, Issue 6, pp. 1301-8, (2014) (PubMed).

    Liu, Gu, Zhao, An, Yang: "Glypican 4 may be involved in the adipose tissue redistribution in high-fat feeding C57BL/6J mice with peroxisome proliferators-activated receptor ? agonist rosiglitazone treatment." in: Experimental and therapeutic medicine, Vol. 8, Issue 6, pp. 1813-1818, (2014) (PubMed).

    Karadag, Ertugrul, Kalkan, Bilgili, Celik, Takci, Balahoroglu, Calka: "The effect of acitretin treatment on insulin resistance, retinol-binding protein-4, leptin, and adiponectin in psoriasis vulgaris: a noncontrolled study." in: Dermatology (Basel, Switzerland), Vol. 227, Issue 2, pp. 103-8, (2013) (PubMed).

    Xie, Yang, Zou, Cheng, Wang, Jiang, Xiao, Wang, Liu: "Influence of the core circadian gene "Clock" on obesity and leptin resistance in mice." in: Brain research, Vol. 1491, pp. 147-55, (2012) (PubMed).

    Yao, Li, Zhao, Zeng, Shen, Xiang, Xiao: "Anti-obesity effect of an isoflavone fatty acid ester on obese mice induced by high fat diet and its potential mechanism." in: Lipids in health and disease, Vol. 9, pp. 49, (2010) (PubMed).

  • 抗原 See all Leptin (LEP) ELISA试剂盒
    Leptin (LEP)
    别名
    LEP (LEP 产品)
    别名
    ob ELISA Kit, obese ELISA Kit, LEPD ELISA Kit, OB ELISA Kit, OBS ELISA Kit, leptin ELISA Kit, Lep ELISA Kit, LEP ELISA Kit, lep ELISA Kit
    背景

    Protein Function: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. .

    Background: Leptin(or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue. Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83 % homologous to the mouse and rat ob proteins, respectively. Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart. Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling. Leptin is a powerful inhibitor of bone formation in vivo. Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q. The standard product used in this kit is recombinant human Leptin.

    Synonyms: Leptin ,Obesity factor ,LEP ,hCG_33000 , tcag7.84 ,

    Full Gene Name: Leptin

    Cellular Localisation: Secreted.
    基因ID
    3952
    UniProt
    A4D0Y8
    途径
    JAK/STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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