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IL-13 ELISA 试剂盒

IL13 适用: 小鼠 AA 26-131 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
产品编号 ABIN411312
发货至: 中国
  • 抗原 See all IL-13 (IL13) ELISA试剂盒
    IL-13 (IL13) (Interleukin 13 (IL13))
    抗原表位
    AA 26-131
    适用
    • 12
    • 5
    • 3
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    15.6-1000 pg/mL
    最低检测浓度
    15.6 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-13
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: E.coli
    Immunogen sequence: S26-F131
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <2pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: E.coli
    Immunogen sequence: S26-F131
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    板类型
    Pre-coated
    实验流程
    mouse IL-13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-13 has been precoated onto 96-well plates. Standards(E.coli, S26-F131) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-13 amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-13 standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 112, Standard deviation: 6.16, CV(%): 5.5
    • Sample 2: n=16, Mean(pg/ml): 355, Standard deviation: 21.3, CV(%): 6
    • Sample 3: n=16, Mean(pg/ml): 628, Standard deviation: 29.52, CV(%): 4.7,
    • Sample 1: n=24, Mean(pg/ml): 149, Standard deviation: 9.24, CV(%): 6.2
    • Sample 2: n=24, Mean(pg/ml): 417, Standard deviation: 29.61, CV(%): 7.1
    • Sample 3: n=24, Mean(pg/ml): 674, Standard deviation: 39.1, CV(%): 5.8
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • Han, Zhang, Tan, Liu, Ma, Ou-Yang, Wu: "EPO modified MSCs can inhibit asthmatic airway remodeling in an animal model." in: Journal of cellular biochemistry, Vol. 119, Issue 1, pp. 1008-1016, (2018) (PubMed).

    Li, Li, Zuo, Ke, Yan, Wen, Zhang, Yang: "T-helper type-2 contact hypersensitivity of Balb/c mice aggravated by dibutyl phthalate via long-term dermal exposure." in: PLoS ONE, Vol. 9, Issue 2, pp. e87887, (2014) (PubMed).

  • 抗原 See all IL-13 (IL13) ELISA试剂盒
    IL-13 (IL13) (Interleukin 13 (IL13))
    别名
    IL13 (IL13 产品)
    别名
    IL13 ELISA Kit, IL-13 ELISA Kit, P600 ELISA Kit, Il-13 ELISA Kit, L13 ELISA Kit, interleukin 13 ELISA Kit, IL13 ELISA Kit, Il13 ELISA Kit
    背景

    Protein Function: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses (By similarity). .

    Background: Interleukin(IL)-13 is a major inducer of fibrosis in many chronic infectious and autoimmune diseases. Recombinant IL-13 protein inhibits inflammatory cytokine production induced by lipopolysaccharide in human peripheral blood monocytes. Moreover, it synergizes with IL-13 in regulating interferon-gamma synthesis in large granular lymphocytes. Interleukin-13 may be critical in regulating inflammatory and immune responses. IL-13 and IL-4 are two cytokines produced by T helper type 2 cells, mast cells, and basophils. In addition to their physiological roles, these cytokines are also implicated in pathological conditions such as asthma and allergy. The genes encoding human IL-4 and IL-13 are located on segment q23-31 of chromosome 5.4 Recombinant mouse IL-13 is a mixture of Ser26-Phe131 amino acids with the molecular mass of 11.5KDa.

    Synonyms: Interleukin-13,IL-13,T-cell activation protein P600,Il13,Il-13,

    Full Gene Name: Interleukin-13

    Cellular Localisation: Secreted.
    基因ID
    16163
    UniProt
    P20109
    途径
    JAK/STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
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