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IL-4 ELISA 试剂盒

Name: IL-4 ELISA 试剂盒
SKU: ABIN411301
Availability: OutOfStock

大鼠 IL-4 ELISA Kit, Colorimetric is an assay for quantification of 大鼠 IL-4, that has been mentioned in 19+ publications.

ELISA

1 image

(19 references)

产品编号 ABIN411301

发货至: 中国

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Quick Overview for IL-4 ELISA 试剂盒 (ABIN411301)

抗原

See all IL-4 (IL4) ELISA试剂盒

IL-4 (IL4) (Interleukin 4 (IL4))

抗原表位

AA 25-147

适用

大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6 pg/mL - 1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum

Discover our top product IL4 ELISA Kit

最低检测浓度

15.6 pg/mL

原理

Sandwich Rat IL-4/Interleukin-4 ELISA Kit to quantitate Rat Il4 in cell culture supernatants and serum.

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: C25-S147
Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

< 1 pg/mL

组件
1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers
试剂未包括

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

样本量

100 μL

板类型

Pre-coated

实验流程

ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

实验精密度

Intra-Assay Precision (CV%): 7%, 6.5%, 4.7%
Inter-Assay Precision (CV%): 7.5%, 6.5%, 5.9%

限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

有效期

12 months
Yang, Liu, Liu, Tan, Lu, Wang, Yan: "Treatment of surgical brain injury by immune tolerance induced by intrathymic and hepatic portal vein injection of brain antigens." in: Scientific reports, Vol. 6, pp. 32030, (2018) (PubMed).

Song, Li, Liang, Dai, Shen, Gong, Wang, Hu, Wang: "Low-level laser facilitates alternatively activated macrophage/microglia polarization and promotes functional recovery after crush spinal cord injury in rats." in: Scientific reports, Vol. 7, Issue 1, pp. 620, (2017) (PubMed).

Li, Hu, Wang, Wu, Zhang: "Different profiles of notch signaling in cigarette smoke-induced pulmonary emphysema and bleomycin-induced pulmonary fibrosis." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 64, Issue 5, pp. 363-71, (2015) (PubMed).

Huang, Wang, Li, Ge, Chen, Chen: "Trichostatin A reduces cisplatin-induced ototoxicity through the STAT6 signaling pathway." in: International journal of molecular medicine, Vol. 36, Issue 2, pp. 493-500, (2015) (PubMed).

Presti, DOrazio, Labra, La Ferla, Mezzasalma, Bizzaro, Giardina, Michelotti, Tursi, Vassallo, Di Gennaro: "Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect." in: Applied microbiology and biotechnology, Vol. 99, Issue 13, pp. 5613-26, (2015) (PubMed).

Qiu, Zhu, Lu, Bai, Liu, Zhao, Sun, Zhang, Li, Liu: "The selective mGluR5 agonist CHPG attenuates SO2-induced oxidative stress and inflammation through TSG-6/NF-?B pathway in BV2 microglial cells." in: Neurochemistry international, Vol. 85-86, pp. 46-52, (2015) (PubMed).

Yuan, Zhang, Yang: "Ligusticum wallichii Extract Inhibited the Expression of IL-1? after AMI in Rats." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 620359, (2014) (PubMed).

Ozkan, Deveci, Kumbak, Simsek, Ilhan, Sekercioglu, Sapmaz: "What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility?" in: Journal of reproductive immunology, Vol. 103, pp. 53-8, (2014) (PubMed).

Jin, Cheng, Liu, Wu, Wang, Wei, Zhou, Qin, Jia, Zhen et al.: "Improvement of functional recovery by chronic metformin treatment is associated with enhanced alternative activation of microglia/macrophages and increased angiogenesis and neurogenesis following ..." in: Brain, behavior, and immunity, Vol. 40, pp. 131-42, (2014) (PubMed).

Ou, Shi, Xu, Tao: "Intranasal immunization with DNA vaccine coexpressing Der p 1 and ubiquitin in an allergic rhinitis mouse model." in: Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, Vol. 113, Issue 6, pp. 658-665.e1, (2014) (PubMed).

Huang, Cao: "The protective effects of Shen-Fu injection on experimental acute pancreatitis in a rat model." in: Oxidative medicine and cellular longevity, Vol. 2014, pp. 248786, (2014) (PubMed).

Li, Li, Zuo, Zhou, Hao, Dang, Jiang, He, Deng: "Up-regulated expression of Tim-3/Gal-9 at maternal-fetal interface in pregnant woman with recurrent spontaneous abortion." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 4, pp. 586-90, (2014) (PubMed).

Yu, Liu, Chen, Zeng: "Anti-inflammatory effect of Qingwen Baidu Decoction (?????) in sepsis rats." in: Chinese journal of integrative medicine, Vol. 20, Issue 12, pp. 934-43, (2014) (PubMed).

Sofian, Aghakhani, Farazi, Banifazl, Eslamifar, Rashidi, Khadem Sadegh, Ramezani: "Serum profile of T helper 1 and T helper 2 cytokines in hepatitis C virus infected patients." in: Hepatitis monthly, Vol. 12, Issue 12, pp. e6156, (2013) (PubMed).

Li, Yu, Ying, Yu: "Antiviral effects of modified dingchuan decoction against respiratory syncytial virus infection in vitro and in an immunosuppressive mouse model." in: Journal of ethnopharmacology, Vol. 147, Issue 1, pp. 238-44, (2013) (PubMed).

Tan, Liu, Yang, Wang, Qin: "An inactivated Pseudomonas aeruginosa medicament inhibits airway allergic inflammation and improves epithelial functions." in: The journal of physiological sciences : JPS, Vol. 63, Issue 1, pp. 63-9, (2012) (PubMed).

Wang, Deng, Jiang, Yao: "The natural plant product sophocarpine ameliorates dextran sodium sulfate-induced colitis in mice by regulating cytokine balance." in: International journal of colorectal disease, Vol. 27, Issue 5, pp. 575-81, (2012) (PubMed).

Zhu, Qiu, Liu, Dong, Weng, Hu, Kuang, Gao, Dan, Tao: "Tolerogenic dendritic cells generated by RelB silencing using shRNA prevent acute rejection." in: Cellular immunology, Vol. 274, Issue 1-2, pp. 12-8, (2012) (PubMed).

Chen, Ni, Ye, Sun, Li, Xu: "Comparison of immunosuppressive activity of stephanoside E and its aglycone from Stephanotis mucronata in vitro." in: International immunopharmacology, Vol. 10, Issue 10, pp. 1153-60, (2010) (PubMed).
抗原 See all IL-4 (IL4) ELISA试剂盒

IL-4 (IL4) (Interleukin 4 (IL4))

别名

IL4

背景

Background: Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-ed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. Rat IL-4 is a 13.5KDa glycoprotein, consisting of 147 amino acids in precursor form and 123 amino acids in mature form.
Gene Full Name: interleukin 4

基因ID

287287

UniProt

P20096

途径

JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation