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IL-2 ELISA 试剂盒

Name: IL-2 ELISA 试剂盒
SKU: ABIN411295
Availability: OutOfStock

人 IL-2 ELISA Kit, Colorimetric is an assay for quantification of 人 IL-2, that has been mentioned in 19+ publications.

ELISA

2 images

(19 references)

产品编号 ABIN411295

发货至: 中国

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Quick Overview for IL-2 ELISA 试剂盒 (ABIN411295)

抗原

See all IL-2 (IL2) ELISA试剂盒

IL-2 (IL2) (Interleukin 2 (IL2))

抗原表位

AA 21-153

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6 pg/mL - 1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Discover our top product IL2 ELISA Kit

最低检测浓度

15.6 pg/mL

原理

Sandwich Human IL-2/Interleukin-2 ELISA Kit to quantitate Human IL2 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: A21-T153
Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

< 1 pg/mL

组件
1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers
试剂未包括

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Interleukin 2 (IL2) ELISA Kit
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Interleukin 2 (IL2) ELISA Kit
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Interleukin 2 (IL2) ELISA Kit
IL2 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

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IL2 适用: 犬 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 2 (IL2) ELISA Kit
IL2 适用: 小鸡 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 2 (IL2) ELISA Kit
IL2 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 2 (IL2) ELISA Kit
IL2 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

样本量

100 μL

板类型

Pre-coated

实验流程

ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

实验精密度

Intra-Assay Precision (CV%): 4.1%, 5.6%, 6.2%
Inter-Assay Precision (CV%): 4.1%, 6.5%, 8.1%

限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

有效期

12 months
Han, Wen, Li, Fan, Zhang, Li, Sun, Geng, Sun, Huang, Wu, Lu, Zheng: "Effect of different anesthetic methods on cellular immune functioning and the prognosis of patients with ovarian cancer undergoing oophorectomy." in: Bioscience reports, Vol. 37, Issue 5, (2018) (PubMed).

Zang, Jiang, Li, DU, Niu: "Characteristics of CD4+CD25+Foxp3+ regulatory T cells in patients with multiple organ dysfunction syndrome." in: Experimental and therapeutic medicine, Vol. 11, Issue 5, pp. 1908-1912, (2016) (PubMed).

Tong, Zhang, Zhao, Liu, Han: "Traffic-related PM2.5 induces cytosolic [Ca²?] increase regulated by Orai1, alters the CaN-NFAT signaling pathway, and affects IL-2 and TNF-? cytoplasmic levels in Jurkat T-cells." in: Archives of environmental contamination and toxicology, Vol. 68, Issue 1, pp. 31-7, (2015) (PubMed).

Hu, Jing, Wang, Zhang, Yong, Wang: "The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D-Galactose-induced aged rat model." in: BMC complementary and alternative medicine, Vol. 15, pp. 119, (2015) (PubMed).

Cheng, Wu, Gao, Xu: "Analysis of influence of baicalin joint resveratrol retention enema on the TNF-?, SIgA, IL-2, IFN-? of rats with respiratory syncytial virus infection." in: Cell biochemistry and biophysics, Vol. 70, Issue 2, pp. 1305-9, (2014) (PubMed).

Yu, Liu, Chen, Zeng: "Anti-inflammatory effect of Qingwen Baidu Decoction (?????) in sepsis rats." in: Chinese journal of integrative medicine, Vol. 20, Issue 12, pp. 934-43, (2014) (PubMed).

Eker, Yavasci, Cangur, Kirli, Sarandol: "Can BDNF and IL-2 be indicators for the diagnosis in schizophrenic patients with depressive symptoms?" in: Acta neuropsychiatrica, Vol. 26, Issue 5, pp. 291-7, (2014) (PubMed).

Liang, Liu, Chen, Sun: "Antitumor activity of the protein and small molecule component fractions from Agrocybe aegerita through enhancement of cytokine production." in: Journal of medicinal food, Vol. 17, Issue 4, pp. 439-46, (2014) (PubMed).

Li, He, Wang, Shu, Liu: "USP2a positively regulates TCR-induced NF-?B activation by bridging MALT1-TRAF6." in: Protein & cell, Vol. 4, Issue 1, pp. 62-70, (2013) (PubMed).

Sofian, Aghakhani, Farazi, Banifazl, Eslamifar, Rashidi, Khadem Sadegh, Ramezani: "Serum profile of T helper 1 and T helper 2 cytokines in hepatitis C virus infected patients." in: Hepatitis monthly, Vol. 12, Issue 12, pp. e6156, (2013) (PubMed).

Liu, Shan, Dong, Liu, Ma, Liu: "Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury." in: World journal of gastroenterology, Vol. 19, Issue 4, pp. 492-502, (2013) (PubMed).

Zhong, Cheng, Long, He, Qi, Xiang, Zhao, Zhu: "Dynamically expressed microRNA-15b modulates the activities of CD8+ T lymphocytes in mice with Lewis lung carcinoma." in: Journal of translational medicine, Vol. 11, pp. 71, (2013) (PubMed).

Sofra, Fei, Fabrizi, Marcelli, Claroni, Gallucci, Ensoli, Forastiere: "Immunomodulatory effects of total intravenous and balanced inhalation anesthesia in patients with bladder cancer undergoing elective radical cystectomy: preliminary results." in: Journal of experimental & clinical cancer research : CR, Vol. 32, pp. 6, (2013) (PubMed).

Zhu, Qiu, Liu, Dong, Weng, Hu, Kuang, Gao, Dan, Tao: "Tolerogenic dendritic cells generated by RelB silencing using shRNA prevent acute rejection." in: Cellular immunology, Vol. 274, Issue 1-2, pp. 12-8, (2012) (PubMed).

Guida, Luongo, Aviello, Palazzo, De Chiaro, Gatta, Boccella, Marabese, Zjawiony, Capasso, Izzo, de Novellis, Maione: "Salvinorin A reduces mechanical allodynia and spinal neuronal hyperexcitability induced by peripheral formalin injection." in: Molecular pain, Vol. 8, pp. 60, (2012) (PubMed).

Liu, Shen, Wei, Mao, Huang: "Immunomodulatory activity of butanol extract from Solanum lyratum in tumor-bearing mice." in: Immunopharmacology and immunotoxicology, Vol. 33, Issue 1, pp. 100-6, (2011) (PubMed).

Xu, Feng, Wang, Zhu, Lin, Lou, Xiang, He, Zheng, Tang, Zuo: "Phytoestrogen calycosin-7-O-?-D-glucopyranoside ameliorates advanced glycation end products-induced HUVEC damage." in: Journal of cellular biochemistry, Vol. 112, Issue 10, pp. 2953-65, (2011) (PubMed).

Chen, Ni, Ye, Sun, Li, Xu: "Comparison of immunosuppressive activity of stephanoside E and its aglycone from Stephanotis mucronata in vitro." in: International immunopharmacology, Vol. 10, Issue 10, pp. 1153-60, (2010) (PubMed).

Li, Liang, Ma, Hu, Yan, Li, Fang, Wang, Zhang, Xie, Zhang: "Calcineurin mediates the immune response of hemocytes through NF-kappaB signaling pathway in pearl oyster (Pinctada fucata)." in: Fish & shellfish immunology, Vol. 28, Issue 2, pp. 253-60, (2010) (PubMed).
抗原 See all IL-2 (IL2) ELISA试剂盒

IL-2 (IL2) (Interleukin 2 (IL2))

别名

IL2

背景

Background: Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4. Human IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.
Gene Full Name: interleukin 2

基因ID

3558

UniProt

P60568

途径

JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation