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IGFBPI ELISA 试剂盒

Name: IGFBPI ELISA 试剂盒
SKU: ABIN411289
Availability: OutOfStock

This 小鼠 IGFBPI ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 IGFBPI. This ELISA Kit has been cited in 3+ publications.

ELISA

1 image

(3 references)

产品编号 ABIN411289

发货至: 中国

Datasheet as PDF

Quick Overview for IGFBPI ELISA 试剂盒 (ABIN411289)

抗原

See all IGFBPI ELISA试剂盒

IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

抗原表位

AA 26-272

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2-2000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Discover our top product IGFBPI ELISA Kit

最低检测浓度

31.2 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IGFBP-1

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: NSO
Immunogen sequence: A26-N272

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<3pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: NSO
Immunogen sequence: A26-N272
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应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Sequence similarities: Contains 1 IGFBP N-terminal domain.

板类型

Pre-coated

实验流程

mouse IGFBP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP-1 has been precoated onto 96-well plates. Standards(NSO, A26-N272) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGFBP-1 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse IGFBP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IGFBP-1 standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 108, Standard deviation: 5.72, CV(%): 5.3
- Sample 2: n=16, Mean(pg/ml): 657, Standard deviation: 28.91, CV(%): 4.4
- Sample 3: n=16, Mean(pg/ml): 1239, Standard deviation: 59.47, CV(%): 4.8,
- Sample 1: n=24, Mean(pg/ml): 115, Standard deviation: 9.09, CV(%): 7.9
- Sample 2: n=24, Mean(pg/ml): 638, Standard deviation: 41.47, CV(%): 6.5
- Sample 3: n=24, Mean(pg/ml): 1341, Standard deviation: 97.89, CV(%): 7.3
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Matz-Soja, Aleithe, Marbach, Böttger, Arnold, Schmidt-Heck, Kratzsch, Gebhardt: "Hepatic Hedgehog signaling contributes to the regulation of IGF1 and IGFBP1 serum levels." in: Cell communication and signaling : CCS, Vol. 12, pp. 11, (2014) (PubMed).

Zhang, Zhao, Hu, Liu, Chen, Chen, Du: "Distinct post-transcriptional regulation of Igfbp1 gene by hypoxia in lowland mouse and Qinghai-Tibet plateau root vole Microtus oeconomus." in: Molecular and cellular endocrinology, Vol. 376, Issue 1-2, pp. 33-42, (2013) (PubMed).

Wang, Wang, Liang, Liu, Shi, Bai, Lin, Magaye, Zhao: "Expression and clinical significance of IGF-1, IGFBP-3, and IGFBP-7 in serum and lung cancer tissues from patients with non-small cell lung cancer." in: OncoTargets and therapy, Vol. 6, pp. 1437-44, (2013) (PubMed).
抗原 See all IGFBPI ELISA试剂盒

IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

别名

IGFBP1

背景

Protein Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration (By similarity). .
Background: IGFBP1 is also known as amniotic fluid binding protein(AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein(IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7. The standard product used in this kit is recombinant mouse IGFBP-1, consisting of 247 amino acids with the molecular mass of 27KDa.
Synonyms: Insulin-like growth factor-binding protein 1,IBP-1,IGF-binding protein 1,IGFBP-1,Igfbp1,Igfbp-1,
Full Gene Name: Insulin-like growth factor-binding protein 1
Cellular Localisation: Secreted.

基因ID

16006

UniProt

P47876

途径

Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding