CX3CL1 ELISA 试剂盒
Quick Overview for CX3CL1 ELISA 试剂盒 (ABIN411273)
抗原
See all CX3CL1 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Fractalkine/CX3CL1
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: Q25-R339 -
交叉反应 （详细）
- There is detectable cross-reactivity with Eotaxin, MCP-1, MCP-2, 6Ckine, MARC< 0.2 % .
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灵敏度
- <50pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: Q25-R339
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Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) ELISA Kit
CX3CL1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) ELISA KitCX3CL1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) ELISA KitCX3CL1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) ELISA KitCX3CL1 适用: 大鼠 AA 25-334 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) ELISA KitCX3CL1 适用: 小鼠 AA 25-337 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) ELISA KitCX3CL1 适用: 大鼠 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) ELISA KitCX3CL1 适用: 小鼠 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Lysates
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) ELISA KitCX3CL1 适用: 大鼠 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) ELISA KitCX3CL1 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- human Fractalkine ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Fractalkine has been precoated onto 96-well plates. Standards(NSO, Q25-R339) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Fractalkine is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fractalkine amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Fractalkine standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine or human milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Fractalkine standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.5, Standard deviation: 0.063, CV(%): 4.2
- Sample 2: n=16, Mean(ng/ml): 3.8, Standard deviation: 0.122, CV(%): 3.2
- Sample 3: n=16, Mean(ng/ml): 6.6, Standard deviation: 0.31, CV(%): 4.7,
- Sample 1: n=24, Mean(ng/ml): 1.3, Standard deviation: 0.111, CV(%): 8.5
- Sample 2: n=24, Mean(ng/ml): 3.7, Standard deviation: 0.237, CV(%): 6.4
- Sample 3: n=24, Mean(ng/ml): 6.5, Standard deviation: 0.403, CV(%): 6.2
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))
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别名
- CX3CL1
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背景
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Background: Chemokine(C-X3-C motif) ligand 1(CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine(in humans) and neurotactin(in mice). CX3CL1 is produced as a long protein(with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble(90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22. It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.
Synonyms: Chemokine (C-X3-C motif) ligand 1 ,cDNA, FLJ94424, Homo sapiens chemokine (C-X3-C motif) ligand 1 (CX3CL1), mRNA ,CX3CL1 ,hCG_15105 ,
Full Gene Name: chemokine (C-X3-C motif) ligand 1
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基因ID
- 6376
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UniProt
- A0N0N7
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途径
- Synaptic Membrane
抗原 See all CX3CL1 ELISA试剂盒
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