FGF9 ELISA 试剂盒
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- 抗原 See all FGF9 (FGF-9) ELISA试剂盒
- FGF9 (FGF-9) (Fibroblast Growth Factor 9 (FGF-9))
- 抗原表位
- AA 1-208
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human FGF9
- 品牌
- PicoKine™
- 样品类型
- Serum, Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: sf21
Immunogen sequence: M1-S208 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 预测反应
- Bovine,Horse,Rabbit
- 灵敏度
- <15pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: sf21
Immunogen sequence: M1-S208 - Top Product
- Discover our top product FGF-9 ELISA Kit
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Fibroblast Growth Factor 9 (FGF-9) ELISA Kit
FGF-9 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 9 (FGF-9) ELISA KitFGF-9 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 9 (FGF-9) ELISA KitFGF-9 适用: 小鸡 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum
Fibroblast Growth Factor 9 (FGF-9) ELISA KitFGF-9 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 9 (FGF-9) ELISA KitFGF-9 适用: Cow Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Fibroblast Growth Factor 9 (FGF-9) ELISA KitSmall Sample FGF-9 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 9 (FGF-9) ELISA KitFGF-9 适用: 人 Colorimetric Sandwich ELISA 200-35000 pg/mL Cell Culture Supernatant, Plasma, Serum
Fibroblast Growth Factor 9 (FGF-9) ELISA KitFGF-9 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 9 (FGF-9) ELISA KitFGF-9 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the heparin-binding growth factors family.
Tissue Specificity: Glial cells.
- 板类型
- Pre-coated
- 实验流程
- human FGF9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF9 has been precoated onto 96-well plates. Standards(sf21, M1-S208) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FGF9 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human FGF9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human FGF9 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 256, Standard deviation: 11.52, CV(%): 4.5
- Sample 2: n=16, Mean(pg/ml): 1145, Standard deviation: 43.51, CV(%): 3.8
- Sample 3: n=16, Mean(pg/ml): 2278, Standard deviation: 75.17, CV(%): 3.3,
- Sample 1: n=24, Mean(pg/ml): 247, Standard deviation: 18.28, CV(%): 7.4
- Sample 2: n=24, Mean(pg/ml): 1242, Standard deviation: 80.73, CV(%): 6.5
- Sample 3: n=24, Mean(pg/ml): 2226, Standard deviation: 135.8, CV(%): 6.1
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all FGF9 (FGF-9) ELISA试剂盒
- FGF9 (FGF-9) (Fibroblast Growth Factor 9 (FGF-9))
- 别名
- FGF9 (FGF-9 产品)
- 背景
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Protein Function: Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors. .
Background: Fibroblast growth factor-9(FGF-9) is a steroid-regulated mitogen and survival factor for nerve and mesenchymal cells. The human FGF-9 cDNA cloned by using oligonucleotide probes encodes a polypeptide consisting of 208 amino acids. Sequence similarity to other members of the FGF family has been estimated to be around 30 % . FGF-9 is an autocrine estromedin endometrial stromal growth factor that plays roles in cyclic proliferation of uterine endometrial stroma. FGF9 is produced and secreted by the prostatic stromal cells. It is a potent mitogen for both prostatic epithelial and stromal cells in culture. FGF9 is an abundant secreted growth factor that can act as both a paracrine mitogen for epithelial cells and an autocrine mitogen for stromal cells. Overexpression of this paracrine and autocrine growth factor may play an important role in the epithelial and stromal proliferation in benign prostatic hyperplasia.4 As a result of glycosylation, the molecular mass is 25-27KDa.
Synonyms: Fibroblast growth factor 9,FGF-9,Glia-activating factor,GAF,Heparin-binding growth factor 9,HBGF-9,FGF9,
Full Gene Name: Fibroblast growth factor 9
Cellular Localisation: Secreted. - 基因ID
- 2254
- UniProt
- P31371
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