BMP2 ELISA 试剂盒
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- 抗原 See all BMP2 ELISA试剂盒
- BMP2 (Bone Morphogenetic Protein 2 (BMP2))
- 抗原表位
- AA 283-396
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat BMP-2
- 品牌
- PicoKine™
- 样品类型
- Osseous Tissue, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: CHO
Immunogen sequence: Q283-R396 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <2pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: CHO
Immunogen sequence: Q283-R396 - Featured
- Discover our best selling BMP2 ELISA Kit
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- Discover our top product BMP2 ELISA Kit
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Bone Morphogenetic Protein 2 (BMP2) ELISA Kit
BMP2 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Bone Morphogenetic Protein 2 (BMP2) ELISA KitBMP2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 2 (BMP2) ELISA KitBMP2 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 2 (BMP2) ELISA KitBMP2 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 2 (BMP2) ELISA KitBMP2 适用: 人 AA 283-396 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Osseous Tissue, Serum
Bone Morphogenetic Protein 2 (BMP2) ELISA KitBMP2 适用: 兔 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 2 (BMP2) ELISA KitBMP2 适用: 小鸡 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 2 (BMP2) ELISA KitBMP2 适用: 犬 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 2 (BMP2) ELISA KitBMP2 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 2 (BMP2) ELISA KitBMP2 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Expressed in femur, calvaria, trachea, lung and ovary.
- 板类型
- Pre-coated
- 实验流程
- rat BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-2 has been precoated onto 96-well plates. Standards(CHO, Q283-R396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat BMP-2 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat BMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat bone tissue, cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat BMP-2 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 349, Standard deviation: 10.1, CV(%): 2.9
- Sample 2: n=16, Mean(pg/ml): 1058, Standard deviation: 33.9, CV(%): 3.2
- Sample 3: n=16, Mean(pg/ml): 2437, Standard deviation: 82.9, CV(%): 3.4,
- Sample 1: n=24, Mean(pg/ml): 357, Standard deviation: 23.2, CV(%): 6.5
- Sample 2: n=24, Mean(pg/ml): 1068, Standard deviation: 75.8, CV(%): 7.1
- Sample 3: n=24, Mean(pg/ml): 2479, Standard deviation: 178.5, CV(%): 7.2
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Thermally induced self-agglomeration 3D scaffolds with BMP-2-loaded core-shell fibers for enhanced osteogenic differentiation of rat adipose-derived stem cells." in: International journal of nanomedicine, Vol. 13, pp. 4145-4155, (2018) (PubMed).
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: "Thermally induced self-agglomeration 3D scaffolds with BMP-2-loaded core-shell fibers for enhanced osteogenic differentiation of rat adipose-derived stem cells." in: International journal of nanomedicine, Vol. 13, pp. 4145-4155, (2018) (PubMed).
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- 抗原 See all BMP2 ELISA试剂盒
- BMP2 (Bone Morphogenetic Protein 2 (BMP2))
- 别名
- BMP2 (BMP2 产品)
- 别名
- BDA2 ELISA Kit, BMP2A ELISA Kit, AI467020 ELISA Kit, Bmp2a ELISA Kit, BMP-2 ELISA Kit, xBMP-2 ELISA Kit, xbmp2 ELISA Kit, BMP2 ELISA Kit, bmp2a ELISA Kit, bmp2 ELISA Kit, wu:fc59d09 ELISA Kit, bone morphogenetic protein 2 ELISA Kit, bone morphogenetic protein 2 L homeolog ELISA Kit, Bone morphogenetic protein 2 ELISA Kit, bone morphogenetic protein 2a ELISA Kit, BMP2 ELISA Kit, Bmp2 ELISA Kit, bmp2.L ELISA Kit, bmp2 ELISA Kit, bmp2a ELISA Kit
- 背景
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Protein Function: Induces cartilage and bone formation.
Background: Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant rat BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
Synonyms: Bone morphogenetic protein 2,BMP-2,Bone morphogenetic protein 2A,BMP-2A,Bmp2,Bmp-2,
Full Gene Name: Bone morphogenetic protein 2
Cellular Localisation: Secreted. - 基因ID
- 29373
- UniProt
- P49001
- 途径
- Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation
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