IgG ELISA 试剂盒
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Quick Overview for IgG ELISA 试剂盒 (ABIN3199222)
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See all IgG ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 3.13 ng/mL
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原理
- This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IgG.
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Analytical Method
- Quantitative
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特异性
- IgG ELISA Kit, human
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组件
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- Plate
- Standard
- Diluent
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试剂未包括
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Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.
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IgG ELISA Kit
适用: 兔 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Rabbit TrueBlot® Set (with IP beads)WB, IP 适用: 兔
Mouse TrueBlot® Set (with IP beads)WB, IP 适用: 小鼠
IgG ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: Cow Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: 小鼠 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA Kit适用: 人 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: 小鸡 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA Kit适用: Pig Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- protect your eyes
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储存条件
- 4 °C
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储存方法
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Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
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- IgG
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物质类
- Antibody
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背景
- Immunoglobulin G (IgG) is a type of antibody. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. IgG is the main type of antibody found in blood and extracellular fluid allowing it to control infection of body tissues. IgG molecules are created and released by plasma B cells. IgG is the only isotype that has receptors to facilitate passage through the human placenta, thereby providing protection to the fetus in utero. IgG antibodies are extracted from donated blood plasma and used as a therapeutic known as intravenous immunoglobulin (IVIG). This is used to treat immune deficiencies, autoimmune disorders, and infections.
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基因ID
- 3500
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