IgA ELISA 试剂盒
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Quick Overview for IgA ELISA 试剂盒 (ABIN3198549)
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See all IgA ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 3.13 ng/mL
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原理
- This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.
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Analytical Method
- Quantitative
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特异性
- IgA ELISA Kit, Human
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组件
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- Plate
- Standard
- Diluent
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试剂未包括
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Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.
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IgA ELISA Kit
适用: Cow Colorimetric Competition ELISA 0.39 μg/mL - 25 μg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
IgA ELISA Kit适用: 绵羊 Colorimetric Competition ELISA 3.75 μg/mL - 60 μg/mL Plasma, Serum
IgA ELISA Kit适用: 小鸡 Colorimetric Competition ELISA 781.25 ng/mL - 50000 ng/mL Plasma, Serum
IgA ELISA Kit适用: 人 Colorimetric Sandwich ELISA 0.14 ng/mL - 100 ng/mL Plasma, Saliva, Serum
IgA ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
IgA ELISA Kit适用: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
IgA ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Plasma, Serum
IgA ELISA Kit适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
IgA ELISA Kit适用: 兔 Colorimetric Sandwich ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
IgA ELISA Kit适用: Pig Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- protect your eyes
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储存条件
- 4 °C
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储存方法
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Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
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- IgA
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物质类
- Antibody
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背景
- Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. Its heavy chains are of the type α. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). In its secretory form, IgA is the main immunoglobulin found in mucous secretions, it is also found in small amounts in blood. The secretory component of sIgA protects the immunoglobulin from being degraded by proteolytic enzymes, thus sIgA can survive in the harsh gastrointestinal tract environment and provide protection against microbes that multiply in body secretions. sIgA can also inhibit inflammatory effects of other immunoglobulins. IgA is a poor activator of the complement system, and opsonises only weakly.
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