BMP7 ELISA 试剂盒
Quick Overview for BMP7 ELISA 试剂盒 (ABIN3071450)
抗原
See all BMP7 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse BMP-7
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: CHO
Immunogen sequence: S292-H430 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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预测反应
- Hamster
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灵敏度
- <10pg/ml
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产品特性
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Protein Name: Bone morphogenetic protein 7
Full Gene Name: bone morphogenetic protein 7 -
试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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Bone Morphogenetic Protein 7 (BMP7) ELISA Kit
BMP7 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: Cow Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: 绵羊 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: 人 AA 293-431 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Osseous Tissue, Plasma (EDTA), Plasma (heparin), Serum, Urine
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: Hamster AA 292-430 Colorimetric Sandwich ELISA
Bone Morphogenetic Protein 7 (BMP7) ELISA KitBMP7 适用: 大鼠 AA 292-430 Colorimetric Sandwich ELISA 31.2-2000 pg/mL
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- A monoclonal antibody from rat specific for BMP-7 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO, Immunogen sequence: S292-H430) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BMP-7 amount of sample captured in plate.
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实验流程
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20-25
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- BMP7 (Bone Morphogenetic Protein 7 (BMP7))
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别名
- BMP7
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背景
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Protein Function: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
Background: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-i² superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.
Synonyms: Bone morphogenetic protein 7,BMP-7,Osteogenic protein 1,OP-1,Bmp7,Bmp-7, Op1,
Full Gene Name: Bone morphogenetic protein 7
Cellular Localisation: Secreted. -
基因ID
- 12162
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UniProt
- P23359
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途径
- Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance
抗原 See all BMP7 ELISA试剂盒
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