APOA1 ELISA 试剂盒
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Quick Overview for APOA1 ELISA 试剂盒 (ABIN3044715)
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 3.12 ng/mL
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原理
- Sandwich Human APOA1 / Apoa I / Apolipoprotein AI ELISA Kit to quantitate Human APOA1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: D25-Q267Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 50 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Apolipoprotein A-I (APOA1) ELISA Kit
APOA1 适用: 人 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 人 Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Cell Culture Supernatant, Plasma, Serum
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 μg/mL - 10 μg/mL Bronchoalveolar Lavage, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 犬 Colorimetric Competition ELISA 28.12 ng/mL - 1800 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: Cow Colorimetric Competition ELISA 78 ng/mL - 5000 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.6%, 7.3%, 5.2%
Inter-Assay Precision (CV%): 6.2%, 7.9%, 6.1% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "Vitreous levels of apolipoprotein A1 and retinol binding protein 4 in human rhegmatogenous retinal detachment associated with choroidal detachment." in: Molecular vision, Vol. 24, pp. 252-260, (2018) (PubMed).
: "Detection of Serum Protein Biomarkers for the Diagnosis and Staging of Hepatoblastoma." in: International journal of molecular sciences, Vol. 16, Issue 6, pp. 12669-85, (2016) (PubMed).
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- APOA1 (Apolipoprotein A-I (APOA1))
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别名
- APOA1
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背景
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Background: Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.
Gene Full Name: apolipoprotein A1
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基因ID
- 335
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UniProt
- P02647
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途径
- Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism
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