APOE ELISA 试剂盒
Quick Overview for APOE ELISA 试剂盒 (ABIN3044714)
抗原
See all APOE ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 3.12 ng/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human APOE
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: K19-H317 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <50pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
- Immunogen sequence: K19-H317
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Apolipoprotein E (APOE) ELISA Kit
APOE 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma
Apolipoprotein E (APOE) ELISA KitAPOE 适用: 大鼠 Colorimetric Competition ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Apolipoprotein E (APOE) ELISA KitAPOE 适用: 小鼠 Colorimetric Competition ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Apolipoprotein E (APOE) ELISA KitAPOE 适用: 人 Colorimetric Sandwich ELISA 4.7 ng/mL - 300 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein E (APOE) ELISA KitAPOE 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein E (APOE) ELISA KitAPOE 适用: 人 Colorimetric Competition ELISA 15.62 ng/mL - 1000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Apolipoprotein E (APOE) ELISA KitAPOE 适用: Pig Colorimetric Competition ELISA 31.25 ng/mL - 2000 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein E (APOE) ELISA KitAPOE 适用: 猴 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein E (APOE) ELISA KitAPOE 适用: 猴 Colorimetric Competition ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Plasma, Saliva, Serum, Tissue Homogenate
Apolipoprotein E (APOE) ELISA KitAPOE 适用: 兔 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Tissue Specificity: Occurs in all lipoprotein fractions in plasma. It constitutes 10-20 % of very low density lipoproteins (VLDL) and 1-2 % of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.
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板类型
- Pre-coated
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实验流程
- human APOE ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for APOE has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: K19-H317) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for APOE is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human APOE amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 200 ng/mL, 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL human APOE standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva, milk or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human APOE standard solution and each sample be measured in duplicate.
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限制
- 仅限研究用
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缓冲液
- heparin or EDTA
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- APOE (Apolipoprotein E (APOE))
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别名
- APOE
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背景
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Protein Function: Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
Background: Apolipoprotein E (APOE) is a class of apolipoprotein found in the chylomicron and Intermediate-density lipoprotein (IDLs) that is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. In peripheral tissues, APOE is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform- dependent manner. In the central nervous system, APOE is mainly produced by astrocytes, and transports cholesterol to neurons via APOE receptors, which are members of the low density lipoprotein receptor gene family. This protein is involved in Alzheimer’s disease and cardiovascular disease.
Synonyms: Apolipoprotein E,Apo-E,APOE,
Full Gene Name: Apolipoprotein E
Cellular Localisation: Secreted. -
基因ID
- 348
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UniProt
- P02649
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途径
- Regulation of Cell Size, Lipid Metabolism
抗原 See all APOE ELISA试剂盒
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