IDS ELISA 试剂盒
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- 抗原 See all IDS ELISA试剂盒
- IDS (Iduronate 2-Sulfatase (IDS))
- 抗原表位
- AA 26-550
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IDS
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: S26-P550 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <15pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
- Immunogen sequence: S26-P550
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Iduronate 2-Sulfatase (IDS) ELISA Kit
IDS 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Iduronate 2-Sulfatase (IDS) ELISA KitIDS 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Iduronate 2-Sulfatase (IDS) ELISA KitIDS 适用: 小鼠 AA 36-552 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Liver, kidney, lung, and placenta.
- 板类型
- Pre-coated
- 实验流程
- human IDS ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IDS has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: S26-P550) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IDS is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IDS amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IDS standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IDS standard solution and each sample be measured in duplicate.
- 限制
- 仅限研究用
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- 缓冲液
- heparin or EDTA
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all IDS ELISA试剂盒
- IDS (Iduronate 2-Sulfatase (IDS))
- 别名
- IDS (IDS 产品)
- 别名
- mps2 ELISA Kit, sids ELISA Kit, zgc:158245 ELISA Kit, MPS2 ELISA Kit, SIDS ELISA Kit, AW214631 ELISA Kit, iduronate 2-sulfatase ELISA Kit, IDS ELISA Kit, CpipJ_CPIJ004938 ELISA Kit, ids ELISA Kit, Ids ELISA Kit
- 背景
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Protein Function: Required for the lysosomal degradation of heparan sulfate and dermatan sulfate.
Background: Iduronate 2-sulfatase (IDS) is a sulfatase enzyme associated with Hunter syndrome. It encodes a member of the sulfatase family of proteins. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Synonyms: Iduronate 2-sulfatase,3.1.6.13,Alpha-L-iduronate sulfate sulfatase,Idursulfase,Iduronate 2-sulfatase 42 kDa chain,Iduronate 2-sulfatase 14 kDa chain,IDS,SIDS,
Full Gene Name: Iduronate 2-sulfatase
Cellular Localisation: Lysosome. - 基因ID
- 3423
- UniProt
- P22304
- 途径
- Glycosaminoglycan Metabolic Process
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