PF4 ELISA 试剂盒
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- 抗原 See all PF4 ELISA试剂盒
- PF4 (Platelet Factor 4 (PF4))
- 抗原表位
- AA 32-101
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL4/PF4
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: E32-S101 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <15pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
- Immunogen sequence: E32-S101
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- Discover our top product PF4 ELISA Kit
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Platelet Factor 4 (PF4) ELISA Kit
PF4 适用: 人 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum
Platelet Factor 4 (PF4) ELISA KitPF4 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Platelet-Poor Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum
Platelet Factor 4 (PF4) ELISA KitPF4 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Platelet-Poor Plasma, Serum
Platelet Factor 4 (PF4) ELISA KitPF4 适用: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma
Platelet Factor 4 (PF4) ELISA KitPF4 适用: Cow Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Cell Lysate, Platelet-Poor Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 适用: 小鼠 AA 30-105 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- human CXCL4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL4 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: E32-S101) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL4 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CXCL4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL4 standard solution and each sample be measured in duplicate.
- 限制
- 仅限研究用
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- 缓冲液
- heparin or EDTA
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all PF4 ELISA试剂盒
- PF4 (Platelet Factor 4 (PF4))
- 别名
- CXCL4 (PF4 产品)
- 别名
- CXCL4 ELISA Kit, PF-4 ELISA Kit, SCYB4 ELISA Kit, Cxcl4 ELISA Kit, Scyb4 ELISA Kit, Pf4a ELISA Kit, RATPF4A ELISA Kit, PF4A ELISA Kit, CXCL4L1 ELISA Kit, CXCL4V1 ELISA Kit, PF4-ALT ELISA Kit, SCYB4V1 ELISA Kit, PF4 ELISA Kit, platelet factor 4 ELISA Kit, platelet factor 4 variant 1 ELISA Kit, PF4 ELISA Kit, Pf4 ELISA Kit, LOC703451 ELISA Kit, PF4V1 ELISA Kit, LOC100524373 ELISA Kit, LOC102181854 ELISA Kit, LOC100630489 ELISA Kit
- 背景
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Protein Function: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. .
Background: Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The PF4 gene was localized on 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
Synonyms: Platelet factor 4,PF-4,C-X-C motif chemokine 4,Iroplact,Oncostatin-A,Platelet factor 4, short form,PF4,CXCL4, SCYB4,
Full Gene Name: Platelet factor 4
Cellular Localisation: Secreted. - 基因ID
- 5196
- UniProt
- P02776
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