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Somatostatin FLISA Kit

This 化学剂 Somatostatin ELISA Kit is a Fluorometric ELISA Kit designed to quantify 化学剂 Somatostatin.
产品编号 ABIN2871228
发货至: 中国

Quick Overview for Somatostatin FLISA Kit (ABIN2871228)

抗原

See all Somatostatin (SST) FLISA Kits
Somatostatin (SST)

适用

  • 5
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
化学剂

检测方法

Fluorometric

实验类型

Competition ELISA

应用范围

ELISA
  • Analytical Method

    Quantitative
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    板类型

    Pre-coated

    实验流程

    The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.

    限制

    仅限研究用
  • 储存条件

    4 °C
  • 抗原 See all Somatostatin (SST) FLISA Kits

    Somatostatin (SST)

    别名

    Somatostatin-28

    物质类

    Chemical
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