CGRP FLISA Kit
Quick Overview for CGRP FLISA Kit (ABIN2871094)
抗原
See all CGRP (CALCA) FLISA Kits适用
检测方法
实验类型
应用范围
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Analytical Method
- Quantitative
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Calcitonin-Related Polypeptide alpha (CALCA) ELISA Kit
CALCA 适用: 人 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 大鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 小鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 小鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 大鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
- The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))
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别名
- Calcitonin Gene Related Peptide (CGRP)
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途径
- Hormone Activity, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, Feeding Behaviour
抗原 See all CGRP (CALCA) FLISA Kits
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