Mitogen-Activated Protein Kinase (MAPK) ELISA 试剂盒
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- 抗原 See all Mitogen-Activated Protein Kinase (MAPK) ELISA试剂盒
- Mitogen-Activated Protein Kinase (MAPK)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- DNA-Binding ELISA
- 应用范围
- ELISA
- 原理
- DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.
- 品牌
- TransAM®
- 样品类型
- Cell Extracts, Tissue Samples
- Analytical Method
- Quantitative
- 特异性
- The TransAM MAPK Family Kit is tested for sensitivity and specificity in detecting MAPK-regulated transcription factor activation.
- 产品特性
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Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. - 组件
- One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.
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Mitogen-Activated Protein Kinase (MAPK) ELISA Kit
MAPK 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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1:1000 dilutions of phosphorylated ATF-2, c-Myc, MEF2 and STAT1a and a 1:500 dilution of phosphorylated c-Jun antibodies are tested using 5 ug/well of nuclear extract prepared from a stimulated cell line: C2C12 for MEF2, HeLa (Anisomycin) for ATF-2, Jurkat (1 day growth) for c-Myc, K-562 (TPA) for c-Jun and U-937 (TPA + IFNg) for STAT1. The relative activation of each family member may vary depending on the cell type tested and the manner in which it was stimulated. TransAM assays are performed in the absense or presence of 20 pmol of competitor oligonucleotide that contains either a wild-type or mutated consensus binding site. The wild-type oligonucleotide prevents binding of the transcription factor to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has a limited effect on transcription factor binding.
- 实验时间
- 5 h
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
- Store the cell extracts at -80°C. All other kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the oligonucleotides, the c-Jun, c-Myc and STAT1 antibodies, DTT and Protease Inhibitor Cocktail that should be kept at -20°C. This product is guaranteed for 6 months from date of receipt.
- 有效期
- 6 months
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- 抗原 See all Mitogen-Activated Protein Kinase (MAPK) ELISA试剂盒
- Mitogen-Activated Protein Kinase (MAPK)
- 别名
- Mapk (MAPK 产品)
- 别名
- Tb06.28P18.710 ELISA Kit, Tb08.10J17.560 ELISA Kit, erk ELISA Kit, erk2 ELISA Kit, ert1 ELISA Kit, mapk ELISA Kit, mapk1 ELISA Kit, mapk1a ELISA Kit, mapk2 ELISA Kit, mpk1 ELISA Kit, p42mapk ELISA Kit, prkm1 ELISA Kit, prkm2 ELISA Kit, xp42 ELISA Kit, mitogen-activated protein kinase ELISA Kit, mitogen-activated protein kinase 1 S homeolog ELISA Kit, Tc00.1047053506229.10 ELISA Kit, Tb927.6.1780 ELISA Kit, Tb927.8.3770 ELISA Kit, mapk1.S ELISA Kit
- 途径
- VEGF Signaling
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