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RNA ChIP-IT Control Kit

ChIP 适用: 人
产品编号 ABIN2866108
发货至: 中国

Quick Overview for RNA ChIP-IT Control Kit (ABIN2866108)

适用

应用范围

Chromatin Immunoprecipitation (ChIP)
  • 品牌

    ChIP-IT®

    特异性

    The RNA ChIP-IT Control Kit - Human is quality-control tested to demonstrate that the positive and negative control antibodies are effective in performing RNA-chromatin immunoprecipitation.
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    说明

    HeLa cells were grown, fixed and used to prepare chromatin as described in the RNA ChIP-IT Kit manual. RNA-ChIP reactions were then performed using 10 μl of Suz12 antibody and 10 μl of control rabbit IgG (0.2 μg/μl). The immunoprecipitated RNA and the control input RNA were then used in RT-PCR using lincSFPQ primer mix. Kit Components: 50 μl Suz12 rabbit polyclonal antibody 100 μl rabbit IgG (0.2 μg/μl) 400 μl lincSFPQ primer mix

    限制

    仅限研究用
  • 储存条件

    -20 °C

    储存方法

    The RNA-ChIP Control Kit - Human components are shipped on dry ice. The lincSFPQ primer mix, the Suz12 antibody and the control rabbit IgG should be stored at -20°C. This product is guaranteed for 6 months from date of receipt under the correct storage conditions. Aliquot the antibodies to avoid exposing to multiple freeze-thaw cycles.

    有效期

    6 months
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