RNA ChIP-IT Control Kit
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- 适用
- 人
- 应用范围
- Chromatin Immunoprecipitation (ChIP)
- 品牌
- ChIP-IT®
- 特异性
- The RNA ChIP-IT Control Kit - Human is quality-control tested to demonstrate that the positive and negative control antibodies are effective in performing RNA-chromatin immunoprecipitation.
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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HeLa cells were grown, fixed and used to prepare chromatin as described in the RNA ChIP-IT Kit manual. RNA-ChIP reactions were then performed using 10 μl of Suz12 antibody and 10 μl of control rabbit IgG (0.2 μg/μl). The immunoprecipitated RNA and the control input RNA were then used in RT-PCR using lincSFPQ primer mix. Kit Components: 50 μl Suz12 rabbit polyclonal antibody 100 μl rabbit IgG (0.2 μg/μl) 400 μl lincSFPQ primer mix
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The RNA-ChIP Control Kit - Human components are shipped on dry ice. The lincSFPQ primer mix, the Suz12 antibody and the control rabbit IgG should be stored at -20°C. This product is guaranteed for 6 months from date of receipt under the correct storage conditions. Aliquot the antibodies to avoid exposing to multiple freeze-thaw cycles.
- 有效期
- 6 months
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