Rapid 大鼠 Monoclonal Antibody Isotyping Kit
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- 适用
- 大鼠
- 最低检测浓度
- 100 ng/mL
- 应用范围
- Isotyping (IsoT)
- 原理
- 5 minute determination of 6 Rat Ig heavy chain isotypes
- 样品类型
- Cell Culture Supernatant, Purified Protein
- 交叉反应 （详细）
- There is no cross-reactivity among each isotype.
- 产品特性
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The Rapid Rat Immunoglobulin Isotyping Kit is a five-minute lateral flow assay with ELISA sensitivity for monoclonal antibody class and subclass determination.
Acceptable sample types include hybridoma culture supernatant and purified monoclonal antibody.
Ascites fluid is not recommended since it usually contains multiple immunoglobulins.
However, this kit can still be used to determine the relative distribution of the main isotypes in a sample of ascites fluid. - 组件
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The isotyping kit has three different sizes with 5, 10 or 20 pouches containing two cassettes per pouch: one cassette is for determining IgG1, IgG2a, IgG2b, IgG2c and the other cassette is for determining Kappa, Lambda, IgA and IgM.
Both strips contain a control line.
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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Lipopolysaccharides (LPS) ELISA Kit适用: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 人 AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 6 (IL6) ELISA KitIL6 适用: 人 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 人 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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1. Invalid result: no red line at ""C"".
2. Negative result: only one red line at ""C"".
3. Positive results:
a). Single monoclonal antibody: In addition to the positive control ""C"" line, only one heavy chain and one light chain will appear.
b). Multiple monoclonal antibodies: In addition to the positive control line, more than one heavy chain and/or one light chain will appear.
The line intensity can be used to judge the dominant Isotypes. - 样本量
- 100 µL
- 实验时间
- 5 min
- 实验流程
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1) Place the cassettes in horizontal plane.
2) Add 80-100 μL antibody solution directly to the Sample Well. Wait for 5 minutes.
3) A red test line at the location corresponding to antibody-specific isotype and a red control line at ""C' position will appear within 1-5 minutes.
NOTE: Do not read after 20 minutes.
NOTE: If the color of tissue culture medium causes high background, 40-60 μL PBS can be chased to clear the background. - 样品制备
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Cell Culture Supernatant: Recommended dilution is 1:10-1:100 in PBS.
Purified Antibody: Dilute samples to 0.5 - 2 μg/mL in PBS
Ascites: Ascites fluid is not recommended since it usually contains multiple Igs. However, this kit can still be used to determine the relative distribution of the main isotypes in a sample of ascites fluid. The recommended dilution is 1:10,000 in PBS. - 限制
- 仅限研究用
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- 注意事项
- Promptly return unused strips in sealed bag and keep dry.
- 储存条件
- RT
- 储存方法
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Store the strips in their original package at room temperature.
This product is stable for up to 18 months from the certified date. Freezing the strips is not recommended. - 有效期
- 18 months
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