ADAM9 ELISA 试剂盒
Quick Overview for ADAM9 ELISA 试剂盒 (ABIN2859343)
抗原
See all ADAM9 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ADAM9
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: A29-D697 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: A29-D697
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ADAM Metallopeptidase Domain 9 (ADAM9) ELISA Kit
ADAM9 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
ADAM Metallopeptidase Domain 9 (ADAM9) ELISA KitADAM9 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Ascitic Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
ADAM Metallopeptidase Domain 9 (ADAM9) ELISA KitADAM9 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
ADAM Metallopeptidase Domain 9 (ADAM9) ELISA KitADAM9 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Contains 1 disintegrin domain.
Tissue Specificity: Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart. .
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板类型
- Pre-coated
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实验流程
- human ADAM9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM9 has been precoated onto 96-well plates. Standards(NSO, A29-D697) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAM9 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human ADAM9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ADAM9 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 145, Standard deviation: 7.54, CV(%): 5.2
- Sample 2: n=16, Mean(pg/ml): 363, Standard deviation: 20.69, CV(%): 5.7
- Sample 3: n=16, Mean(pg/ml): 594, Standard deviation: 36.23, CV(%): 6.1,
- Sample 1: n=24, Mean(pg/ml): 176, Standard deviation: 11.44, CV(%): 6.5
- Sample 2: n=24, Mean(pg/ml): 380, Standard deviation: 28.12, CV(%): 7.4
- Sample 3: n=24, Mean(pg/ml): 658, Standard deviation: 51.98, CV(%): 7.9
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- ADAM9 (ADAM Metallopeptidase Domain 9 (ADAM9))
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别名
- ADAM9
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背景
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Protein Function: Probable zinc protease. May mediate cell-cell or cell- matrix interactions. Isoform 2 displays alpha-secretase activity for APP. .
Background: Disintegrin and metalloproteinase domain-containing protein 9, also known as MCMP or MDC9 is an enzyme that in humans is encoded by the ADAM9 gene. This gene encodes a member of the ADAM('a disintegrin and metalloproteinase') family and exhibits a number of characteristics of class III reprolysins. This gene is mapped to 8p11.22. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis.
Synonyms: Disintegrin and metalloproteinase domain-containing protein 9,ADAM 9,3.4.24.-,Cellular disintegrin-related protein,Meltrin-gamma,Metalloprotease/disintegrin/cysteine-rich protein 9,Myeloma cell metalloproteinase,ADAM9,KIAA0021, MCMP, MDC9, MLTNG,
Full Gene Name: Disintegrin and metalloproteinase domain-containing protein 9
Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein. -
基因ID
- 8754
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UniProt
- Q13443
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途径
- Cellular Response to Molecule of Bacterial Origin, SARS-CoV-2 Protein Interactome
抗原 See all ADAM9 ELISA试剂盒
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