Syndecan 1 ELISA 试剂盒
Quick Overview for Syndecan 1 ELISA 试剂盒 (ABIN2859341)
抗原
See all Syndecan 1 (SDC1) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 156 pg/mL
-
原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human SDC1/Syndecan-1
-
品牌
- PicoKine™
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: NSO
Immunogen sequence: Q23-G254 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- <10pg/mL
-
试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
-
免疫原
-
Expression system for standard: NSO
Immunogen sequence: Q23-G254
-
-
Syndecan 1 (SDC1) ELISA Kit
SDC1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Syndecan 1 (SDC1) ELISA KitSDC1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Syndecan 1 (SDC1) ELISA KitSDC1 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Syndecan 1 (SDC1) ELISA KitHigh Sensitivity SDC1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Syndecan 1 (SDC1) ELISA KitSDC1 适用: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Syndecan 1 (SDC1) ELISA KitSDC1 适用: 小鼠 AA 18-252 Colorimetric Sandwich ELISA
Syndecan 1 (SDC1) ELISA KitSDC1 适用: 兔 Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum
-
-
应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
-
说明
-
Sequence similarities: Belongs to the syndecan proteoglycan family.
-
板类型
- Pre-coated
-
实验流程
- human Syndecan-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Syndecan-1 has been precoated onto 96-well plates. Standards(NSO, Q23-G254) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Syndecan-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Syndecan-1 amount of sample captured in plate.
-
实验流程
-
Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Syndecan-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Syndecan-1 standard solution and each sample be measured in duplicate.
-
实验精密度
-
- Sample 1: n=16, Mean(ng/ml): 1.23, Standard deviation: 0.048, CV(%): 3.9
- Sample 2: n=16, Mean(ng/ml): 3.25, Standard deviation: 0.15, CV(%): 4.6
- Sample 3: n=16, Mean(ng/ml): 5.87, Standard deviation: 0.299, CV(%): 5.1,
- Sample 1: n=24, Mean(ng/ml): 1.32, Standard deviation: 0.07, CV(%): 5.3
- Sample 2: n=24, Mean(ng/ml): 4.05, Standard deviation: 0.235, CV(%): 5.8
- Sample 3: n=24, Mean(ng/ml): 6.42, Standard deviation: 0.417, CV(%): 6.5
-
限制
- 仅限研究用
-
-
-
注意事项
- Avoid multiple freeze-thaw cycles.
-
储存条件
- -20 °C,4 °C
-
储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
-
有效期
- 12 months
-
-
- Syndecan 1 (SDC1)
-
别名
- SDC1
-
背景
-
Protein Function: Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.
Background: Syndecan 1, also known as SYND1 or CD138, is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane(type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. This gene is mapped to 2p24.1. The syndecans mediate cell binding, cell signaling, cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan 1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. What’s more, Syndecan-1 is also a sponge for growth factors, with binding largely via heparan sulfate chains.
Synonyms: Syndecan-1,SYND1,CD138,SDC1,SDC,
Full Gene Name: Syndecan-1
Cellular Localisation: Membrane, Single-pass type I membrane protein. Secreted. Shedding of the ectodomain produces a soluble form. -
基因ID
- 6382
-
UniProt
- P18827
-
途径
- Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism
抗原 See all Syndecan 1 (SDC1) ELISA试剂盒
-
