TIMP4 ELISA 试剂盒
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- 抗原 See all TIMP4 ELISA试剂盒
- TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))
- 抗原表位
- AA 30-224
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78-5.000 pg/mL
- 最低检测浓度
- 78 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TIMP-4
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: C30-P224 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: C30-P224 - Featured
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TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA Kit
TIMP4 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA KitTIMP4 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA KitTIMP4 适用: 兔 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA KitTIMP4 适用: 小鼠 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum
TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA KitTIMP4 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA KitTIMP4 适用: 兔 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum
TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA KitTIMP4 适用: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA KitTIMP4 适用: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA KitTIMP4 适用: 人 Colorimetric Sandwich ELISA 25-7000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family.
Tissue Specificity: Expressed in brain, heart, ovary and skeletal muscle.
- 板类型
- Pre-coated
- 实验流程
- mouse TIMP-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TIMP-4 has been precoated onto 96-well plates. Standards(NSO, C30-P224) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TIMP-4 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 5,000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL mouse TIMP-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse TIMP-4 standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 272, Standard deviation: 12.24, CV(%): 4.5
- Sample 2: n=16, Mean(pg/ml): 830, Standard deviation: 473.1, CV(%): 5.7
- Sample 3: n=16, Mean(pg/ml): 1934, Standard deviation: 98.6, CV(%): 5.1,
- Sample 1: n=24, Mean(pg/ml): 296, Standard deviation: 22.5, CV(%): 7.6
- Sample 2: n=24, Mean(pg/ml): 793, Standard deviation: 53.92, CV(%): 6.8
- Sample 3: n=24, Mean(pg/ml): 1615, Standard deviation: 116.3, CV(%): 7.2
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all TIMP4 ELISA试剂盒
- TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))
- 别名
- TIMP4 (TIMP4 产品)
- 别名
- TIMP4 ELISA Kit, TIMP-4 ELISA Kit, timp4 ELISA Kit, TIMP metallopeptidase inhibitor 4 ELISA Kit, TIMP metallopeptidase inhibitor 4, tandem duplicate 3 ELISA Kit, tissue inhibitor of metalloproteinase 4 ELISA Kit, TIMP4 ELISA Kit, timp4.3 ELISA Kit, Timp4 ELISA Kit
- 背景
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Protein Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
Background: Metalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. TIMP4 gene is mapped to chromosome 3p25. TIMP4 gene contains 5 exons that span 6 kb of genomic DNA. The gene is expressed as a 1.4-kb transcript abundant in heart and present at low levels in several other tissues. Expressed TIMP4 inhibits MMPs in vitro. What’s more, TIMP-4 binds both progelatinase A and the C domain in a similar manner to that of TIMP-2.
Synonyms: Metalloproteinase inhibitor 4,Tissue inhibitor of metalloproteinases 4,TIMP-4,Timp4,
Full Gene Name: Metalloproteinase inhibitor 4
Cellular Localisation: Secreted. - 基因ID
- 110595
- UniProt
- Q9JHB3
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