IGF2R ELISA 试剂盒

Sequence similarities: Belongs to the MRL1/IGF2R family.

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IGF2R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGF2R standard solution and each sample be measured in duplicate.

• Sample 1: n=16, Mean(ng/ml): 1.8, Standard deviation: 0.063, CV(%): 3.5
• Sample 2: n=16, Mean(ng/ml): 4.2, Standard deviation: 0.172, CV(%): 4.1
• Sample 3: n=16, Mean(ng/ml): 6.7, Standard deviation: 0.389, CV(%): 5.8,
• Sample 1: n=24, Mean(ng/ml): 1.5, Standard deviation: 0.065, CV(%): 4.3
• Sample 2: n=24, Mean(ng/ml): 3.9, Standard deviation: 0.222, CV(%): 5.7
• Sample 3: n=24, Mean(ng/ml): 6.4, Standard deviation: 0.397, CV(%): 6.2

Protein Function: Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6- phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. .

Background: Insulin-like growth factor 2 receptor, also called IGF2R or I-MPR is a protein that in humans is encoded by the IGF2R gene. This gene is mapped to 6q25.3. This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the maternal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the maternal allele.

Synonyms: Cation-independent mannose-6-phosphate receptor,CI Man-6-P receptor,CI-MPR,M6PR,300 kDa mannose 6-phosphate receptor,MPR 300,Insulin-like growth factor 2 receptor,Insulin-like growth factor II receptor,IGF-II receptor,M6P/IGF2 receptor,M6P/IGF2R,CD222,IGF2R,MPRI,

Full Gene Name: Cation-independent mannose-6-phosphate receptor