TNC ELISA 试剂盒
Quick Overview for TNC ELISA 试剂盒 (ABIN2859318)
抗原
See all TNC ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 93.7 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Tenascin-C/TNC
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
- NSO, G23-P625
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: G23-P625
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Tenascin C (TNC) ELISA Kit
TNC 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tenascin C (TNC) ELISA KitTNC 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Tenascin C (TNC) ELISA KitTNC 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Tenascin C (TNC) ELISA KitTNC 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Tenascin C (TNC) ELISA KitTNC 适用: 小鼠 Colorimetric Competition ELISA 9.4 pg/mL - 600 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- human Tenascin-C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Tenascin-C has been precoated onto 96-well plates. Standards(NSO,G23-P625) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Tenascin-C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Tenascin-C amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m human Tenascin-C standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Tenascin-C standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 843, Standard deviation: 43.8, CV(%): 5.2
- Sample 2: n=16, Mean(pg/ml): 2186, Standard deviation: 118.0, CV(%): 5.4
- Sample 3: n=16, Mean(pg/ml): 3627, Standard deviation: 192.7, CV(%): 5.9,
- Sample 1: n=24, Mean(pg/ml): 924, Standard deviation: 53.5, CV(%): 5.8
- Sample 2: n=24, Mean(pg/ml): 2570, Standard deviation: 159.3, CV(%): 6.2
- Sample 3: n=24, Mean(pg/ml): 4085, Standard deviation: 249.1, CV(%): 6.1
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- TNC (Tenascin C (TNC))
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别名
- TNC
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背景
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Protein Function: Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
Background: Tenascin C (TN-C) is a glycoprotein that in humans is encoded by the TNC gene. It is expressed in the extracellular matrix of various tissues during development, disease or injury, and in restricted neurogenic areas of the central nervous system. Tenascin-C is the founding member of the gene family (Tenascin). In the embryo it is made by migrating cells like the neural crest, it is also abundant in developing tendons, bone and cartilage. TN-C clearly plays a role in cell signaling as evidenced by its ability to be induced during events such as trauma, inflammation, or cancer development. Also, TN-C is important in regulating cell proliferation and migration, especially during developmental differentiation and wound healing.
Synonyms: Tenascin,TN,Cytotactin,GMEM,GP 150-225,Glioma-associated-extracellular matrix antigen,Hexabrachion,JI,Myotendinous antigen,Neuronectin,Tenascin-C,TN-C,TNC,HXB,
Full Gene Name: Tenascin
Cellular Localisation: Secreted, extracellular space, extracellular matrix. -
基因ID
- 3371
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UniProt
- P24821
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途径
- Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development
抗原 See all TNC ELISA试剂盒
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