PDGF-BB Homodimer ELISA 试剂盒
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- 抗原
- PDGF-BB Homodimer
- 抗原表位
- AA 82-190
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- For quantitative detection of human PDGF-BB in cell culture supernates, serum and plasma(heparin, EDTA).
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
- Natural and recombinant human PDGF-BB
- 交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 组件
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- 96-well plate precoated with anti- human PDGF-BB antibod - 1
- Lyophilized recombinant human PDGF-BB standar - 10ng/tubex2
- Biotinylated anti- human PDGF-BB antibod - 130μl(dilution 1:100)
- Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
- Sample diluent buffe - 30 ml
- Antibody diluent buffe - 12ml
- ABC diluent buffe - 12ml
- TMB color developing agen - 10ml
- TMB stop solutio - 10ml
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: S82-T190 - Featured
- Discover our best selling PDGFB ELISA Kit
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
- human PDGF-BB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PDGF-BB has been precoated onto 96-well plates. Standards(E.coli, S82-T190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PDGF-BB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PDGF-BB amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human PDGF-BB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human PDGF-BB standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 342, Standard deviation: 18.5, CV(%): 5.4
- Sample 2: n=16, Mean(pg/ml): 818, Standard deviation: 38.5, CV(%): 4.7
- Sample 3: n=16, Mean(pg/ml): 1163, Standard deviation: 71, CV(%): 6.1,
- Sample 1: n=24, Mean(pg/ml): 326, Standard deviation: 25.43, CV(%): 7.8
- Sample 2: n=24, Mean(pg/ml): 794, Standard deviation: 60.4, CV(%): 7.6
- Sample 3: n=24, Mean(pg/ml): 1234, Standard deviation: 105, CV(%): 8.5
- 限制
- 仅限研究用
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- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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SARI inhibits angiogenesis and tumour growth of human colon cancer through directly targeting ceruloplasmin." in: Nature communications, Vol. 7, pp. 11996, (2018) (PubMed).
: "Expression and functional analysis of platelet-derived growth factor in uterine leiomyomata." in: Cancer biology & therapy, Vol. 5, Issue 1, pp. 28-33, (2006) (PubMed).
: "
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SARI inhibits angiogenesis and tumour growth of human colon cancer through directly targeting ceruloplasmin." in: Nature communications, Vol. 7, pp. 11996, (2018) (PubMed).
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- 抗原
- PDGF-BB Homodimer
- 别名
- PDGF-BB
- 别名
- PDGF-2 ELISA Kit, PDGF2 ELISA Kit, SIS ELISA Kit, SSV ELISA Kit, c-sis ELISA Kit, PDGF-B ELISA Kit, Sis ELISA Kit, platelet derived growth factor subunit B ELISA Kit, platelet derived growth factor, B polypeptide ELISA Kit, PDGFB ELISA Kit, Pdgfb ELISA Kit
- 背景
- Platelet-derived growth factor subunit B is a protein that in humans is encoded by the PDGFB gene. The protein encoded by this gene is a member of the platelet-derived growth factor family. This gene product can exist either as a homodimer(PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide(PDGF-AB), where the dimers are connected by disulfide bonds. This gene is mapped to 22q13.1. Growth factor plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. This gene plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.
- 基因ID
- 5155
- UniProt
- P01127
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