PDGF-BB Homodimer ELISA 试剂盒
(2 references)Quick Overview for PDGF-BB Homodimer ELISA 试剂盒 (ABIN2859308)
抗原
抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- For quantitative detection of human PDGF-BB in cell culture supernates, serum and plasma(heparin, EDTA).
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
- Natural and recombinant human PDGF-BB
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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组件
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- 96-well plate precoated with anti- human PDGF-BB antibod - 1
- Lyophilized recombinant human PDGF-BB standar - 10ng/tubex2
- Biotinylated anti- human PDGF-BB antibod - 130μl(dilution 1:100)
- Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
- Sample diluent buffe - 30 ml
- Antibody diluent buffe - 12ml
- ABC diluent buffe - 12ml
- TMB color developing agen - 10ml
- TMB stop solutio - 10ml
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: S82-T190
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PDGF-BB Homodimer ELISA Kit
适用: 大鼠 Colorimetric Sandwich ELISA 39.063 pg/mL - 2500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
PDGF-BB Homodimer ELISA Kit适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
PDGF-BB Homodimer ELISA Kit适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
PDGF-BB Homodimer ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
PDGF-BB Homodimer ELISA Kit适用: 猴 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
PDGF-BB Homodimer ELISA Kit适用: 兔 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
PDGF-BB Homodimer ELISA Kit适用: Cow Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
PDGF-BB Homodimer ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 39.06 pg/mL - 2500 pg/mL Cell Culture Supernatant, Plasma, Serum
PDGF-BB Homodimer ELISA Kit适用: 马 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
PDGF-BB Homodimer ELISA KitSmall Sample 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Platelet-Poor Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
- human PDGF-BB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PDGF-BB has been precoated onto 96-well plates. Standards(E.coli, S82-T190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PDGF-BB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PDGF-BB amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human PDGF-BB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human PDGF-BB standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 342, Standard deviation: 18.5, CV(%): 5.4
- Sample 2: n=16, Mean(pg/ml): 818, Standard deviation: 38.5, CV(%): 4.7
- Sample 3: n=16, Mean(pg/ml): 1163, Standard deviation: 71, CV(%): 6.1,
- Sample 1: n=24, Mean(pg/ml): 326, Standard deviation: 25.43, CV(%): 7.8
- Sample 2: n=24, Mean(pg/ml): 794, Standard deviation: 60.4, CV(%): 7.6
- Sample 3: n=24, Mean(pg/ml): 1234, Standard deviation: 105, CV(%): 8.5
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限制
- 仅限研究用
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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: "SARI inhibits angiogenesis and tumour growth of human colon cancer through directly targeting ceruloplasmin." in: Nature communications, Vol. 7, pp. 11996, (2018) (PubMed).
: "Expression and functional analysis of platelet-derived growth factor in uterine leiomyomata." in: Cancer biology & therapy, Vol. 5, Issue 1, pp. 28-33, (2006) (PubMed).
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: "SARI inhibits angiogenesis and tumour growth of human colon cancer through directly targeting ceruloplasmin." in: Nature communications, Vol. 7, pp. 11996, (2018) (PubMed).
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- PDGF-BB Homodimer
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别名
- PDGF-BB
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背景
- Platelet-derived growth factor subunit B is a protein that in humans is encoded by the PDGFB gene. The protein encoded by this gene is a member of the platelet-derived growth factor family. This gene product can exist either as a homodimer(PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide(PDGF-AB), where the dimers are connected by disulfide bonds. This gene is mapped to 22q13.1. Growth factor plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. This gene plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.
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基因ID
- 5155
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UniProt
- P01127
抗原
-
