IL-25 ELISA 试剂盒
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- 抗原 See all IL-25 (IL25) ELISA试剂盒
- IL-25 (IL25) (Interleukin 25 (IL25))
- 抗原表位
- AA 33-177
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-17E/IL-25
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: Y33-G177 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: Y33-G177 - Featured
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Interleukin 25 (IL25) ELISA Kit
IL25 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Interleukin 25 (IL25) ELISA KitIL25 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 25 (IL25) ELISA KitIL25 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 25 (IL25) ELISA KitIL25 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 25 (IL25) ELISA KitIL25 适用: 小鼠 Colorimetric Sandwich ELISA 0.03-2.0 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 25 (IL25) ELISA KitIL25 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the IL-17 family.
Tissue Specificity: Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.
- 板类型
- Pre-coated
- 实验流程
- human IL-17E ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17E has been precoated onto 96-well plates. Standards(E.coli, Y33-G177) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17E is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17E amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IL-17E standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human IL-17E standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 621, Standard deviation: 24.2, CV(%): 3.9
- Sample 2: n=16, Mean(pg/ml): 1969, Standard deviation: 90.6, CV(%): 4.6
- Sample 3: n=16, Mean(pg/ml): 2795, Standard deviation: 150.9, CV(%): 5.4,
- Sample 1: n=24, Mean(pg/ml): 730, Standard deviation: 30.7, CV(%): 4.2
- Sample 2: n=24, Mean(pg/ml): 2103, Standard deviation: 119.9, CV(%): 5.7
- Sample 3: n=24, Mean(pg/ml): 3065, Standard deviation: 208.4, CV(%): 6.8
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Effect of n-3 and n-6 unsaturated fatty acids on prostate cancer (PC-3) and prostate epithelial (RWPE-1) cells in vitro." in: Lipids in health and disease, Vol. 12, pp. 160, (2014) (PubMed).
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: "Effect of n-3 and n-6 unsaturated fatty acids on prostate cancer (PC-3) and prostate epithelial (RWPE-1) cells in vitro." in: Lipids in health and disease, Vol. 12, pp. 160, (2014) (PubMed).
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- 抗原 See all IL-25 (IL25) ELISA试剂盒
- IL-25 (IL25) (Interleukin 25 (IL25))
- 别名
- IL25 (IL25 产品)
- 别名
- IL17E ELISA Kit, IL-17e ELISA Kit, Il17e ELISA Kit, IL25 ELISA Kit, RGD1561632 ELISA Kit, interleukin 25 ELISA Kit, IL25 ELISA Kit, Il25 ELISA Kit
- 背景
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Protein Function: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
Background: Interleukin-25 (IL-25), also known as interleukin-17E (IL-17E), is a protein that in humans is encoded by the IL25 gene. It is a cytokine that belongs to the IL-17 cytokine family and mapped to 14q11.2. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. It has been found that IL-25 can induce Th2-type cytokine expression and production by non-T, non-B accessory cells that express high levels of major histocompatibility complex class II and low levels of Cd11c. This cytokine also can induce NF-i°B activation, and stimulate the production of IL8. What’s more, IL-25 is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract.
Synonyms: Interleukin-25,IL-25,Interleukin-17E,IL-17E,IL25,IL17E,UNQ3120/PRO10272,
Full Gene Name: Interleukin-25
Cellular Localisation: Secreted. - 基因ID
- 64806
- UniProt
- Q9H293
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