IL17C ELISA 试剂盒
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- 抗原 See all IL17C ELISA试剂盒
- IL17C (Interleukin 17C (IL17C))
- 抗原表位
- AA 17-194
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 125-8000 pg/mL
- 最低检测浓度
- 125 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-17C
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: D17-Q194 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: D17-Q194 - Featured
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- Discover our top product IL17C ELISA Kit
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Interleukin 17C (IL17C) ELISA Kit
IL17C 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 17C (IL17C) ELISA KitIL17C 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 17C (IL17C) ELISA KitIL17C 适用: 人 AA 19-197 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 17C (IL17C) ELISA KitIL17C 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Interleukin 17C (IL17C) ELISA KitIL17C 适用: 小鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the IL-17 family.
Tissue Specificity: Expressed by epithelial cells after bacterial challenge. Low expression, if any, in lymphocytes. .
- 板类型
- Pre-coated
- 实验流程
- mouse IL-17C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17C has been precoated onto 96-well plates. Standards(E.coli, D17-Q194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-17C amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse IL-17C standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-17C standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.3, Standard deviation: 0.053, CV(%): 4.1
- Sample 2: n=16, Mean(ng/ml): 3.8, Standard deviation: 0.186, CV(%): 4.9
- Sample 3: n=16, Mean(ng/ml): 5.7, Standard deviation: 0.296, CV(%): 5.2,
- Sample 1: n=24, Mean(ng/ml): 1.6, Standard deviation: 0.086, CV(%): 5.4
- Sample 2: n=24, Mean(ng/ml): 4.5, Standard deviation: 0.279, CV(%): 6.2
- Sample 3: n=24, Mean(ng/ml): 6.3, Standard deviation: 0.422, CV(%): 6.7
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all IL17C ELISA试剂盒
- IL17C (Interleukin 17C (IL17C))
- 别名
- IL17C (IL17C 产品)
- 别名
- IL17C ELISA Kit, il17-3 ELISA Kit, CX2 ELISA Kit, IL-17C ELISA Kit, IL-21 ELISA Kit, interleukin 17C ELISA Kit, interleukin 17c ELISA Kit, IL17C ELISA Kit, il17c ELISA Kit, Il17c ELISA Kit
- 背景
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Protein Function: Cytokine that plays a crucial role in innate immunity of the epithelium, including to intestinal bacterial pathogens, in an autocrine manner. Stimulates the production of antibacterial peptides and proinflammatory molecules for host defense by signaling through the NFKB and MAPK pathways. Acts synergically with IL22, TNF and IL1B in inducing antibacterial peptides. May have protective function by maintaining epithelial homeostasis after an inflammatory challenge, such as that caused in the intestine by dextran sulfate sodium in a colitis model. May also promote an inflammatory phenotype, such as skin in a psoriasis model. Enhanced IL17C/IL17RE signaling may also lead to greater susceptibility to autoimmune diseases, such as autoimmune encephalitis. .
Background: IL17C, also known as CX2, is a protein that in humans is encoded by the IL17C gene. IL17C is mapped to 16q24.3. The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. IL17C is an essential autocrine cytokine regulating innate epithelial immune responses. It also plays an important role in the pathogenesis of inflammatory arthritis.
Synonyms: Interleukin-17C,Il-17c,Cytokine CX2,Il17c,
Full Gene Name: Interleukin-17C
Cellular Localisation: Secreted. - 基因ID
- 234836
- UniProt
- Q8K4C5
- 途径
- Cellular Response to Molecule of Bacterial Origin
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