Cathepsin L ELISA 试剂盒
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- 抗原 See all Cathepsin L (CTSL1) ELISA试剂盒
- Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))
- 抗原表位
- AA 113-333
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Cathepsin L
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
- NSO, E113-V333
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: E113-V333 - Featured
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Cathepsin L1 (CTSL1) ELISA Kit
CTSL1 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathepsin L1 (CTSL1) ELISA KitCTSL1 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Cathepsin L1 (CTSL1) ELISA KitCTSL1 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cathepsin L1 (CTSL1) ELISA KitCTSL1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Cathepsin L1 (CTSL1) ELISA KitCTSL1 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathepsin L1 (CTSL1) ELISA KitCTSL1 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathepsin L1 (CTSL1) ELISA KitCTSL1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
SensoLyte® Rh110 Cathepsin L Assay KitFRET Fluorometric
Cathepsin L1 (CTSL1) ELISA KitCTSL1 适用: 人 Colorimetric Sandwich ELISA 24-6.000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- human Cathepsin L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cathepsin L has been precoated onto 96-well plates. Standards(NSO, E113-V333) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cathepsin L amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Cathepsin L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Cathepsin L standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 477, Standard deviation: 27.7, CV(%): 5.8
- Sample 2: n=16, Mean(pg/ml): 1541, Standard deviation: 92.46, CV(%): 6
- Sample 3: n=16, Mean(pg/ml): 2626, Standard deviation: 128.7, CV(%): 4.9,
- Sample 1: n=24, Mean(pg/ml): 527, Standard deviation: 32.15, CV(%): 6.1
- Sample 2: n=24, Mean(pg/ml): 1712, Standard deviation: 114.7, CV(%): 6.7
- Sample 3: n=24, Mean(pg/ml): 3015, Standard deviation: 168.84, CV(%): 5.6
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all Cathepsin L (CTSL1) ELISA试剂盒
- Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))
- 别名
- CTSL (CTSL1 产品)
- 背景
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Protein Function: Important for the overall degradation of proteins in lysosomes.
Background: Cathepsin L is an important lysosomal endopeptidase enzyme which is involved in the initiation of protein degradation. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. The gene is mapped to 9q21-q22. It is a member of the Peptidase C1 family, which play an important role in diverse processes including normal lysosome mediated protein turnover, antigen and proprotein processing, and apoptosis. Cathepsin L has been reported in many organisms including fish, birds and mammals.
Synonyms: Cathepsin L1,3.4.22.15,Cathepsin L,Major excreted protein,MEP,Cathepsin L1 heavy chain,Cathepsin L1 light chain,CTSL,CTSL1,
Full Gene Name: Cathepsin L1
Cellular Localisation: Lysosome. - 基因ID
- 1514
- UniProt
- P07711
- 途径
- Activation of Innate immune Response, Toll-Like Receptors Cascades
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