ADAM8 ELISA 试剂盒
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- 抗原 See all ADAM8 ELISA试剂盒
- ADAM8 (ADAM Metallopeptidase Domain 8 (ADAM8))
- 抗原表位
- AA 1-497
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ADAM8
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- NSO, M1-P497
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: M1-P497 - Top Product
- Discover our top product ADAM8 ELISA Kit
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ADAM Metallopeptidase Domain 8 (ADAM8) ELISA Kit
ADAM8 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
ADAM Metallopeptidase Domain 8 (ADAM8) ELISA KitADAM8 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
ADAM Metallopeptidase Domain 8 (ADAM8) ELISA KitADAM8 适用: 人 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
ADAM Metallopeptidase Domain 8 (ADAM8) CLIA KitADAM8 适用: 人 Chemiluminescent Sandwich ELISA 15.625 pg/mL - 1000 pg/mL
ADAM Metallopeptidase Domain 8 (ADAM8) ELISA KitADAM8 适用: 小鼠 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Plasma, Serum, Tissue Homogenate
ADAM Metallopeptidase Domain 8 (ADAM8) ELISA KitADAM8 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Expressed on neutrophils and monocytes.
- 板类型
- Pre-coated
- 实验流程
- human ADAM8 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM8 has been precoated onto 96-well plates. Standards(NSO, M1-P497) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAM8 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human ADAM8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ADAM8 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 0.22, Standard deviation: 0.013, CV(%): 5.7
- Sample 2: n=16, Mean(ng/ml): 1.47, Standard deviation: 0.090, CV(%): 6.1
- Sample 3: n=16, Mean(ng/ml): 2.34, Standard deviation: 0.185, CV(%): 7.9,
- Sample 1: n=24, Mean(ng/ml): 0.27, Standard deviation: 0.018, CV(%): 6.8
- Sample 2: n=24, Mean(ng/ml): 1.56, Standard deviation: 0.115, CV(%): 7.4
- Sample 3: n=24, Mean(ng/ml): 2.58, Standard deviation: 0.214, CV(%): 8.3
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all ADAM8 ELISA试剂盒
- ADAM8 (ADAM Metallopeptidase Domain 8 (ADAM8))
- 别名
- ADAM8 (ADAM8 产品)
- 背景
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Protein Function: Possible involvement in extravasation of leukocytes.
Background: A Disintegrin and metalloproteinase domain-containing protein 8 is an enzyme that in humans is encoded by the ADAM8 gene. ADAM8 is localized to chromosome 10q26.3. This gene encodes a member of the ADAM (a disintegrin and metalloproteinase domain) family. Members of the ADAM family, such as ADAM8, are cell surface proteases involved in remodeling of extracellular matrix, cell migration, and processing of membrane-bound signaling molecules. They are characterized by disintegrin and metalloprotease domains that confer adhesive properties and proteolytic activities, respectively. And the protein encoded by this gene may be involved in cell adhesion duringneurodegeneration.
Synonyms: Disintegrin and metalloproteinase domain-containing protein 8,ADAM 8,3.4.24.-,Cell surface antigen MS2,CD156a,ADAM8,MS2,
Full Gene Name: Disintegrin and metalloproteinase domain-containing protein 8
Cellular Localisation: Membrane, Single-pass type I membrane protein. - 基因ID
- 101
- UniProt
- P78325
- 途径
- Activation of Innate immune Response, M Phase
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