Leptin ELISA 试剂盒
(12 references)Quick Overview for Leptin ELISA 试剂盒 (ABIN2859273)
抗原
See all Leptin (LEP) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- For quantitative detection of mouse Leptin in cell culture supernates, serum and plasma(heparin, EDTA).
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
- Natural and recombinant mouse Leptin
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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组件
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- 96-well plate precoated with anti- mouse Leptin antibod - 1
- Lyophilized recombinant mouse Leptin standar - 10ng/tubex2
- Biotinylated anti- mouse Leptin antibod - 130ul(dilution 1:100)
- Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
- Sample diluent buffe - 30ml
- Antibody diluent buffe - 12ml
- ABC diluent buffe - 12ml
- TMB color developing agen - 10ml
- TMB stop solutio - 10ml
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: V22-C167
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Leptin (LEP) ELISA Kit
LEP 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: 人 Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: 兔 Colorimetric Competition ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: 兔 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: 小鸡 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Leptin (LEP) ELISA KitLEP 适用: Salmon Colorimetric Competition ELISA 62.5 pg/mL - 1000 pg/mL Plasma, Serum
Leptin (LEP) ELISA KitLEP 适用: Pig Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
- mouse Leptin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Leptin has been precoated onto 96-well plates. Standards(E.coli, V22-C167) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Leptin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Leptin amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Leptin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Leptin standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 196, Standard deviation: 8.82, CV(%): 4.5
- Sample 2: n=16, Mean(pg/ml): 1123, Standard deviation: 58.4, CV(%): 5.2
- Sample 3: n=16, Mean(pg/ml): 2253, Standard deviation: 135.2, CV(%): 6,
- Sample 1: n=24, Mean(pg/ml): 216, Standard deviation: 12.1, CV(%): 5.6
- Sample 2: n=24, Mean(pg/ml): 1339, Standard deviation: 83.02, CV(%): 6.2
- Sample 3: n=24, Mean(pg/ml): 2487, Standard deviation: 174.1, CV(%): 7
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限制
- 仅限研究用
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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: "Beneficial Effects of Red Yeast Rice on High-Fat Diet-Induced Obesity, Hyperlipidemia, and Fatty Liver in Mice." in: Journal of medicinal food, Vol. 18, Issue 10, pp. 1095-102, (2015) (PubMed).
: "A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes." in: Natural products and bioprospecting, Vol. 5, Issue 3, pp. 159-66, (2015) (PubMed).
: "Phylloquinone supplementation improves glycemic status independent of the effects of adiponectin levels in premonopause women with prediabetes: a double-blind randomized controlled clinical trial." in: Journal of diabetes and metabolic disorders, Vol. 14, Issue 1, pp. 1, (2015) (PubMed).
: "Effect of Lactobacillus plantarum Strain K21 on High-Fat Diet-Fed Obese Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 391767, (2015) (PubMed).
: "Hyperleptinemia directly affects testicular maturation at different sexual stages in mice, and suppressor of cytokine signaling 3 is involved in this process." in: Reproductive biology and endocrinology : RB&E, Vol. 12, pp. 15, (2014) (PubMed).
: "Beneficial effects of phosphatidylcholine on high-fat diet-induced obesity, hyperlipidemia and fatty liver in mice." in: Life sciences, Vol. 118, Issue 1, pp. 7-14, (2014) (PubMed).
: "Glypican 4 may be involved in the adipose tissue redistribution in high-fat feeding C57BL/6J mice with peroxisome proliferators-activated receptor ? agonist rosiglitazone treatment." in: Experimental and therapeutic medicine, Vol. 8, Issue 6, pp. 1813-1818, (2014) (PubMed).
: "Whole-grain intake favorably affects markers of systemic inflammation in obese children: a randomized controlled crossover clinical trial." in: Molecular nutrition & food research, Vol. 58, Issue 6, pp. 1301-8, (2014) (PubMed).
: "Leptin and IL-8: two novel cytokines screened out in childhood lead exposure." in: Toxicology letters, Vol. 227, Issue 3, pp. 172-8, (2014) (PubMed).
: "The effect of acitretin treatment on insulin resistance, retinol-binding protein-4, leptin, and adiponectin in psoriasis vulgaris: a noncontrolled study." in: Dermatology (Basel, Switzerland), Vol. 227, Issue 2, pp. 103-8, (2013) (PubMed).
: "Influence of the core circadian gene "Clock" on obesity and leptin resistance in mice." in: Brain research, Vol. 1491, pp. 147-55, (2012) (PubMed).
: "Anti-obesity effect of an isoflavone fatty acid ester on obese mice induced by high fat diet and its potential mechanism." in: Lipids in health and disease, Vol. 9, pp. 49, (2010) (PubMed).
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: "Beneficial Effects of Red Yeast Rice on High-Fat Diet-Induced Obesity, Hyperlipidemia, and Fatty Liver in Mice." in: Journal of medicinal food, Vol. 18, Issue 10, pp. 1095-102, (2015) (PubMed).
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- Leptin (LEP)
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别名
- LEP
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背景
- Leptin(or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue. Mouse ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83 % homologous to the mouse and rat ob proteins, respectively. Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart. Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling. Leptin is a powerful inhibitor of bone formation in vivo. Comparative mapping of mouse and mouse DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of mouse chromosome 7q. The standard product used in this kit is recombinant mouse Leptin.
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基因ID
- 16846
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UniProt
- P41160
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途径
- JAK/STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
抗原 See all Leptin (LEP) ELISA试剂盒
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