CD73 ELISA 试剂盒
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Quick Overview for CD73 ELISA 试剂盒 (ABIN2859267)
抗原
See all CD73 (NT5E) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Human CD73/NT5E/Ecto-5'-nucleotidase ELISA Kit to quantitate Human NT5E in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: CHO
Immunogen sequence: W27-K547Capture antibody and Detection antibody: polyclonal antibody from sheep, polyclonal antibody from sheep
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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5'-Nucleotidase, Ecto (CD73) (NT5E) ELISA Kit
NT5E 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
5'-Nucleotidase, Ecto (CD73) (NT5E) ELISA KitNT5E 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
5'-Nucleotidase, Ecto (CD73) (NT5E) ELISA KitNT5E 适用: 小鼠 Colorimetric Sandwich ELISA 0.125 mU/mL - 8 mU/mL Plasma, Serum, Tissue Homogenate
5'-Nucleotidase, Ecto (CD73) (NT5E) ELISA KitNT5E 适用: 大鼠 Colorimetric Sandwich ELISA 3.125 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
5'-Nucleotidase, Ecto (CD73) (NT5E) ELISA KitNT5E 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
5'-Nucleotidase, Ecto (CD73) (NT5E) ELISA KitNT5E 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
5'-Nucleotidase, Ecto (CD73) (NT5E) ELISA KitNT5E 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
5'-Nucleotidase, Ecto (CD73) (NT5E) ELISA KitNT5E 适用: 人 Colorimetric Sandwich ELISA 3.12-200 ng/mL Plasma, Serum, Tissue Homogenate
5'-Nucleotidase, Ecto (CD73) (NT5E) ELISA KitNT5E 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 6.7%, 4.1%, 7.2%
Inter-Assay Precision (CV%): 8.4%, 5.3%, 7.3% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
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别名
- NT5E
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背景
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Background: 5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.
Gene Full Name: 5'-nucleotidase ecto
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基因ID
- 4907
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UniProt
- Q6NZX3
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途径
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
抗原 See all CD73 (NT5E) ELISA试剂盒
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