CD47 ELISA 试剂盒

Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Tissue Specificity: Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.

Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human CD47 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD47 standard solution and each sample be measured in duplicate.

• Sample 1: n=16, Mean(pg/ml): 347, Standard deviation: 17, CV(%): 4.9
• Sample 2: n=16, Mean(pg/ml): 1200, Standard deviation: 66, CV(%): 5.5
• Sample 3: n=16, Mean(pg/ml): 5260, Standard deviation: 326.12, CV(%): 6.2,
• Sample 1: n=24, Mean(pg/ml): 450, Standard deviation: 28.8, CV(%): 6.4
• Sample 2: n=24, Mean(pg/ml): 1823, Standard deviation: 123.96, CV(%): 6.8
• Sample 3: n=24, Mean(pg/ml): 5896, Standard deviation: 442.2, CV(%): 7.5

Protein Function: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. .

Background: CD47 (Cluster of Differentiation 47), also known as integrin associated protein (IAP), is a transmembrane protein that in humans is encoded by the CD47 gene. It belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPi±). CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. Also CD47 is ubiquitously expressed in human cells and has found to be overexpressed in many different tumor cells.

Synonyms: Leukocyte surface antigen CD47,Antigenic surface determinant protein OA3,Integrin-associated protein,IAP,Protein MER6,CD47,CD47,MER6,

Full Gene Name: Leukocyte surface antigen CD47