TDGF1 ELISA 试剂盒
Quick Overview for TDGF1 ELISA 试剂盒 (ABIN2859256)
抗原
See all TDGF1 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Cripto/TDGF1
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
- NSO, R26-Q150
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: R26-Q150
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Teratocarcinoma-Derived Growth Factor 1 (TDGF1) ELISA Kit
TDGF1 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Teratocarcinoma-Derived Growth Factor 1 (TDGF1) ELISA KitTDGF1 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Teratocarcinoma-Derived Growth Factor 1 (TDGF1) ELISA KitTDGF1 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Tissue Specificity: Expressed at low level in specific organs of the adult animal such as spleen, heart, lung and brain. During gastrulation, expressed in the forming mesoderm. In later stages of the developing heart, expression is restricted to the truncus arteriosus.
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板类型
- Pre-coated
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实验流程
- mouse Cripto ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cripto has been precoated onto 96-well plates. Standards (NSO, R26-Q150) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cripto is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Cripto amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Cripto standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Cripto standard solution and each sample is measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 675, Standard deviation: 34.42, CV(%): 5.1
- Sample 2: n=16, Mean(pg/ml): 1810, Standard deviation: 108.6, CV(%): 6.0
- Sample 3: n=16, Mean(pg/ml): 2944, Standard deviation: 197.24, CV(%): 6.7,
- Sample 1: n=24, Mean(pg/ml): 792, Standard deviation: 43.56, CV(%): 5.5
- Sample 2: n=24, Mean(pg/ml): 1948, Standard deviation: 140.25, CV(%): 7.2
- Sample 3: n=24, Mean(pg/ml): 3107, Standard deviation: 211.27, CV(%): 6.8
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))
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别名
- TDGF1
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背景
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Protein Function: Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
Background: Teratocarcinoma-derived growth factor 1 is a protein that in humans is encoded by the TDGF1 gene. It is mapped to 3p23-p21. The protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic developmentand tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants.
Synonyms: Teratocarcinoma-derived growth factor,Cripto growth factor,Epidermal growth factor-like Cripto protein,Tdgf1,Cripto,
Full Gene Name: Teratocarcinoma-derived growth factor
Cellular Localisation: Cell membrane, Lipid-anchor, GPI-anchor. -
基因ID
- 21667
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UniProt
- P51865
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途径
- EGFR Signaling Pathway
抗原 See all TDGF1 ELISA试剂盒
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