Stanniocalcin 1 ELISA 试剂盒

Tissue Specificity: Expressed in most tissues, with the highest levels in ovary, prostate, heart, kidney and thyroid. In the kidney, expression is confined to the nephron, specifically in the distal convoluted tubule and in the collecting tubule. Not detected in the brain, liver, spleen, peripheral blood leukocytes and adrenal medulla.

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human STC1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human STC1 standard solution and each sample be measured in duplicate.

• Sample 1: n=16, Mean(pg/ml): 246, Standard deviation: 14.02, CV(%): 5.7
• Sample 2: n=16, Mean(pg/ml): 801, Standard deviation: 48.06, CV(%): 6.0
• Sample 3: n=16, Mean(pg/ml): 1351, Standard deviation: 74.30, CV(%): 5.5,
• Sample 1: n=24, Mean(pg/ml): 254, Standard deviation: 15.74, CV(%): 6.2
• Sample 2: n=24, Mean(pg/ml): 775, Standard deviation: 49.6, CV(%): 6.4
• Sample 3: n=24, Mean(pg/ml): 1416, Standard deviation: 67.96, CV(%): 6.8

Protein Function: Stimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia.

Background: Stanniocalcin-1 is a glycoprotein which is encoded by the STC1 gene. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. Human Stanniocalcin-1 is a putative molecular biomarker of leukemic microenvironment and the only molecular function known up to date is a SUMO E3 ligase activity in the SUMOylation cycle. STC1 interacts with lots of proteins in the cytoplasm, mitochondria, endoplasmatic reticulum and dot-like fashion in the nucleus. The N-terminal region of STC1 is the function region which is responsible to establish the interaction with its partners, including SUMO1. Low resolution studies shows that STC1 is an anti-parallel homodimer in solution and the cystein 202 is responsible for the dimerization of this protein. All the 5 dissulfide bonds of human STC1 are conserved and have the same profile of fish STC.

Synonyms: Stanniocalcin-1,STC-1,STC1,STC,

Full Gene Name: Stanniocalcin-1