ADGRE5 ELISA 试剂盒

Tissue Specificity: Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompanied by detectable levels of soluble CD97 in the synovial fluid.

Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human CD97 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD97 standard solution and each sample be measured in duplicate.

• Sample 1: n=16, Mean(pg/ml): 199, Standard deviation: 11.54, CV(%): 5.8
• Sample 2: n=16, Mean(pg/ml): 894, Standard deviation: 52.74, CV(%): 5.9
• Sample 3: n=16, Mean(pg/ml): 2165, Standard deviation: 134.23, CV(%): 6.2,
• Sample 1: n=24, Mean(pg/ml): 201, Standard deviation: 12.86, CV(%): 6.4
• Sample 2: n=24, Mean(pg/ml): 715, Standard deviation: 47.19, CV(%): 6.6
• Sample 3: n=24, Mean(pg/ml): 3013, Standard deviation: 213.92, CV(%): 7.1

Protein Function: Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration (By similarity). .

Background: Cluster of differentiation 97 is a protein also known as BL-Ac [F2] encoded by the ADGRE5 gene. The CD97 gene is mapped to 19p13.2-p13.12 by fluorescence in situ hybridization. In the immune system, CD97 is known as a critical mediator of host defense. Upon lymphoid, myeloid cells and neutrophil activation, CD97 is upregulated to promote adhesion and migration to sites of inflammation. Moreover, it has been shown that CD97 regulates granulocyte homeostasis. CD97 is expressed at the sarcoplasmic reticulum and the peripheral sarcolemma in skeletal muscle. However, lack of CD97 only affects the structure of the sarcoplasmic reticulum, but not the function of skeletal muscle.

Synonyms: CD97 antigen,Leukocyte antigen CD97,CD97,CD97 antigen subunit alpha,CD97 antigen subunit beta,CD97,

Full Gene Name: CD97 antigen