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PLTP ELISA 试剂盒

PLTP 适用: 小鼠 AA 18-493 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
产品编号 ABIN2859237
发货至: 中国
  • 抗原 See all PLTP ELISA试剂盒
    PLTP (Phospholipid Transfer Protein (PLTP))
    抗原表位
    AA 18-493
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.78-50 ng/mL
    最低检测浓度
    0.78 ng/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse PLTP
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: NSO
    Immunogen sequence: E18-A493
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <20pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: NSO
    Immunogen sequence: E18-A493
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Tissue Specificity: Highest in lung, adipose tissue, brain, and heart.

    板类型
    Pre-coated
    实验流程
    mouse PLTP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PLTP has been precoated onto 96-well plates. Standards(NSO, E18-A493) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLTP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse PLTP amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse PLTP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse PLTP standard solution and each sample is measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(ng/ml): 9.4, Standard deviation: 0.498, CV(%): 5.3
    • Sample 2: n=16, Mean(ng/ml): 18.5, Standard deviation: 0.907, CV(%): 4.9
    • Sample 3: n=16, Mean(ng/ml): 27, Standard deviation: 1.134, CV(%): 4.2,
    • Sample 1: n=24, Mean(ng/ml): 11.2, Standard deviation: 0.75, CV(%): 6.7
    • Sample 2: n=24, Mean(ng/ml): 17.9, Standard deviation: 0.931, CV(%): 5.2
    • Sample 3: n=24, Mean(ng/ml): 31, Standard deviation: 1.49, CV(%): 4.8
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • 抗原 See all PLTP ELISA试剂盒
    PLTP (Phospholipid Transfer Protein (PLTP))
    别名
    PLTP (PLTP 产品)
    别名
    zgc:100903 ELISA Kit, MGC84445 ELISA Kit, PLTP ELISA Kit, hdlcq9 ELISA Kit, DKFZp469A1437 ELISA Kit, BPIFE ELISA Kit, HDLCQ9 ELISA Kit, Bpife ELISA Kit, OD107 ELISA Kit, phospholipid transfer protein ELISA Kit, phospholipid transfer protein S homeolog ELISA Kit, PLTP ELISA Kit, pltp ELISA Kit, pltp.S ELISA Kit, LOC542343 ELISA Kit, Pltp ELISA Kit
    背景

    Protein Function: Facilitates the transfer of a spectrum of different lipid molecules, including diacylglycerol, phosphatidic acid, sphingomyelin, phosphatidylcholine, phosphatidylglycerol, cerebroside and phosphatidyl ethanolamine. Essential for the transfer of excess surface lipids from triglyceride-rich lipoproteins to HDL, thereby facilitating the formation of smaller lipoprotein remnants, contributing to the formation of LDL, and assisting in the maturation of HDL particles. PLTP also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma: an active form that can transfer PC from phospholipid vesicles to high-density lipoproteins (HDL), and an inactive form that lacks this capability (By similarity). .

    Background: Phospholipid transfer protein (PLTP), also known as lipid transfer protein II is a protein that in humans is encoded by the PLTP gene. This gene is mapped to 20q13.12. The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene.

    Synonyms: Phospholipid transfer protein,Lipid transfer protein II,Pltp,

    Full Gene Name: Phospholipid transfer protein

    Cellular Localisation: Secreted.
    基因ID
    18830
    UniProt
    P55065
    途径
    Lipid Metabolism
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