CEACAM1 ELISA 试剂盒
Quick Overview for CEACAM1 ELISA 试剂盒 (ABIN2859230)
抗原
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CEACAM1
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: Q35-G428 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: Q35-G428
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Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) ELISA Kit
CEACAM1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) ELISA KitCEACAM1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) ELISA KitCEACAM1 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) ELISA KitCEACAM1 适用: 人 Colorimetric Sandwich ELISA 20-15000 pg/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the immunoglobulin superfamily. CEA family.
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板类型
- Pre-coated
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实验流程
- human CEACAM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CEACAM1 has been precoated onto 96-well plates. Standards(NSO, Q35-G428) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CEACAM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CEACAM1 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human CEACAM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CEACAM1 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 5.14, Standard deviation: 0.185, CV(%): 3.6
- Sample 2: n=16, Mean(ng/ml): 7.35, Standard deviation: 0.397, CV(%): 5.4
- Sample 3: n=16, Mean(ng/ml): 12.1, Standard deviation: 0.593, CV(%): 4.9,
- Sample 1: n=24, Mean(ng/ml): 5.36, Standard deviation: 0.295, CV(%): 5.5
- Sample 2: n=24, Mean(ng/ml): 8.04, Standard deviation: 0.571, CV(%): 7.1
- Sample 3: n=24, Mean(ng/ml): 13.5, Standard deviation: 0.837, CV(%): 6.2
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))
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别名
- CEACAM1
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背景
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Background: CEACAM1, also known as CD66a (Cluster of Differentiation 66a), is a human glycoprotein encoded by a gene. This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. CEACAM1 is mapped to 19q13.2 in human. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses.
Synonyms: Carcinoembryonic antigen-related cell adhesion molecule 1,Biliary glycoprotein 1,BGP-1,CD66a,CEACAM1,BGP, BGP1,
Full Gene Name: Carcinoembryonic antigen-related cell adhesion molecule 1
Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein. -
基因ID
- 634
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UniProt
- P13688
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