Biglycan ELISA 试剂盒
Quick Overview for Biglycan ELISA 试剂盒 (ABIN2859228)
抗原
See all Biglycan (BGN) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Biglycan
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: D38-K368 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: D38-K368
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Biglycan (BGN) ELISA Kit
BGN 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Biglycan (BGN) ELISA KitBGN 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Biglycan (BGN) ELISA KitBGN 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Biglycan (BGN) ELISA KitBGN 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Biglycan (BGN) ELISA KitBGN 适用: 小鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Biglycan (BGN) ELISA KitBGN 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Tissue Specificity: Found in several connective tissues, especially in articular cartilages.
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板类型
- Pre-coated
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实验流程
- human Biglycan ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Biglycan has been precoated onto 96-well plates. Standards(NSO, D38-K368) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Biglycan is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Biglycan amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Biglycan standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Biglycan standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.62, Standard deviation: 0.06, CV(%): 3.7
- Sample 2: n=16, Mean(ng/ml): 2.91, Standard deviation: 0.157, CV(%): 5.4
- Sample 3: n=16, Mean(ng/ml): 5.38, Standard deviation: 0.264, CV(%): 4.9,
- Sample 1: n=24, Mean(ng/ml): 1.52, Standard deviation: 0.062, CV(%): 4.1
- Sample 2: n=24, Mean(ng/ml): 3.07, Standard deviation: 0.209, CV(%): 6.8
- Sample 3: n=24, Mean(ng/ml): 6.34, Standard deviation: 0.33, CV(%): 5.2
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- Biglycan (BGN)
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别名
- BGN
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背景
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Protein Function: May be involved in collagen fiber assembly. .
Background: Biglycan (BGN) is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. It is an important structural component of articular cartilage and participates in the assembly of the chondrocyte extracellular matrix through formation of protein interactions with type VI collagen and large proteoglycan aggregates. In humans, Biglycan is encoded by the BGN gene. It is mapped to Xq28. Biglycan is believed to play a role in the mineralisation of bone, and its core protein binds to the growth factors BMP-4 and influences its bioactivity. It has been reported that the presence of biglycan is necessary for BMP-4 to exert its effects on osteoblasts. There is also evidence that biglycan can bind to TGF-beta 1. In addition, Biglycan plays an important role in cell signaling.
Synonyms: Biglycan,Bone/cartilage proteoglycan I,PG-S1,BGN,SLRR1A,
Full Gene Name: Biglycan
Cellular Localisation: Secreted, extracellular space, extracellular matrix. -
基因ID
- 633
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UniProt
- P21810
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途径
- Glycosaminoglycan Metabolic Process
抗原 See all Biglycan (BGN) ELISA试剂盒
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