SDC4 ELISA 试剂盒
Quick Overview for SDC4 ELISA 试剂盒 (ABIN2859227)
抗原
See all SDC4 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 62.5 pg/mL
-
原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Syndecan-4
-
品牌
- PicoKine™
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: NSO
Immunogen sequence: E19-E145 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- <10pg/mL
-
试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
-
免疫原
-
Expression system for standard: NSO
Immunogen sequence: E19-E145
-
-
Syndecan 4 (SDC4) ELISA Kit
SDC4 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Syndecan 4 (SDC4) ELISA KitSDC4 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Syndecan 4 (SDC4) ELISA KitSDC4 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Syndecan 4 (SDC4) ELISA KitSDC4 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Syndecan 4 (SDC4) ELISA KitSDC4 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
-
-
应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
-
说明
-
Tissue Specificity: Expressed in epithelial and fibroblastic cells. .
-
板类型
- Pre-coated
-
实验流程
- human Syndecan-4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Syndecan-4 has been precoated onto 96-well plates. Standards(NSO, E19-E145) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Syndecan-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Syndecan-4 amount of sample captured in plate.
-
实验流程
-
Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Syndecan-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Syndecan-4 standard solution and each sample be measured in duplicate.
-
实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 630, Standard deviation: 26.5, CV(%): 4.2
- Sample 2: n=16, Mean(pg/ml): 1447, Standard deviation: 83.9, CV(%): 5.8
- Sample 3: n=16, Mean(pg/ml): 2135, Standard deviation: 100.3, CV(%): 4.7,
- Sample 1: n=24, Mean(pg/ml): 718, Standard deviation: 35.2, CV(%): 4.9
- Sample 2: n=24, Mean(pg/ml): 1604, Standard deviation: 104.3, CV(%): 6.5
- Sample 3: n=24, Mean(pg/ml): 2369, Standard deviation: 120.8, CV(%): 5.1
-
限制
- 仅限研究用
-
-
-
注意事项
- Avoid multiple freeze-thaw cycles.
-
储存条件
- -20 °C,4 °C
-
储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
-
有效期
- 12 months
-
-
- SDC4 (Syndecan 4 (SDC4))
-
别名
- SDC4
-
背景
-
Protein Function: Cell surface proteoglycan that bears heparan sulfate.
Background: Syndecan-4 (SDC4) is a protein that in humans is encoded by the SDC4 gene. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. It is mapped to 20q13.12. Syndecan-4 is one of the four vertebrate syndecans and has a molecular weight of ~20 kDa. It is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. Syndecan-4 also regulates the actin cytoskeleton, cell adhesion, and cell migration. In addition, Syndecan-4 can activate protein kinase C (PKC). The variable domain of syndecan-4 could be a site of self-association. What’s more, Syndecan-4 also binds to phosphatidylinositol (4,5)-bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold.
Synonyms: Syndecan-4,SYND4,Amphiglycan,Ryudocan core protein,SDC4,
Full Gene Name: Syndecan-4
Cellular Localisation: Isoform 1: Membrane, Single-pass type I membrane protein. Secreted. Shedding of the ectodomain produces a soluble form.. -
基因ID
- 6385
-
UniProt
- P31431
-
途径
- Glycosaminoglycan Metabolic Process
抗原 See all SDC4 ELISA试剂盒
-
