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EPO ELISA 试剂盒

EPO 适用: 大鼠 AA 44-226 Colorimetric Sandwich ELISA 46.9-3000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum, Tissue Homogenate
产品编号 ABIN2859223
发货至: 中国
  • 抗原 See all EPO ELISA试剂盒
    EPO (Erythropoietin (EPO))
    抗原表位
    AA 44-226
    适用
    • 6
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    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    46.9-3000 pg/mL
    最低检测浓度
    46.9 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat EPO
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: E.coli
    Immunogen sequence: T44-K226
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <10pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: E.coli
    Immunogen sequence: T44-K226
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Tissue Specificity: Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

    板类型
    Pre-coated
    实验流程
    rat EPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EPO has been precoated onto 96-well plates. Standards(E.coli, T44-K226) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat EPO amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL,375.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL rat EPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants, serum, plasma(heparin) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat EPO standard solution and each sample is measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 513, Standard deviation: 18.98, CV(%): 3.7
    • Sample 2: n=16, Mean(pg/ml): 1265, Standard deviation: 53.13, CV(%): 4.2
    • Sample 3: n=16, Mean(pg/ml): 1974, Standard deviation: 96.73, CV(%): 4.9,
    • Sample 1: n=24, Mean(pg/ml): 648, Standard deviation: 33.7, CV(%): 5.2
    • Sample 2: n=24, Mean(pg/ml): 1450, Standard deviation: 82.65, CV(%): 5.7
    • Sample 3: n=24, Mean(pg/ml): 2301, Standard deviation: 145, CV(%): 6.3
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • 抗原 See all EPO ELISA试剂盒
    EPO (Erythropoietin (EPO))
    别名
    EPO (EPO 产品)
    别名
    EPO ELISA Kit, EP ELISA Kit, MVCD2 ELISA Kit, erythropoietin ELISA Kit, erythropoietin S homeolog ELISA Kit, erythropoietin a ELISA Kit, EPO ELISA Kit, epo ELISA Kit, epo.S ELISA Kit, Epo ELISA Kit, epoa ELISA Kit
    物质类
    Hormone
    背景

    Protein Function: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

    Background: Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. This gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What’s more, EPO is also involved in the wound healing process.

    Synonyms: Erythropoietin,Epo,

    Full Gene Name: Erythropoietin

    Cellular Localisation: Secreted.
    基因ID
    24335
    UniProt
    P29676
    途径
    JAK/STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
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