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MMP8 ELISA 试剂盒

Name: MMP8 ELISA 试剂盒
SKU: ABIN2859213
Availability: OutOfStock

This 大鼠 MMP8 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 大鼠 MMP8. This ELISA Kit has been cited in1 publication.

ELISA

1 image

(1 reference)

产品编号 ABIN2859213

发货至: 中国

Quick Overview for MMP8 ELISA 试剂盒 (ABIN2859213)

抗原

See all MMP8 ELISA试剂盒

MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

抗原表位

AA 21-466

适用

大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

156-10.000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin)

Discover our top product MMP8 ELISA Kit

最低检测浓度

156 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat MMP-8

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: NSO
Immunogen sequence: L21-P466

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<10pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: NSO
Immunogen sequence: L21-P466
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MMP8 适用: 豚鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate

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MMP8 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

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Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA Kit
MMP8 适用: 人 AA 21-467 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Saliva, Serum

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Sequence similarities: Belongs to the peptidase M10A family.
Tissue Specificity: Can degrade fibrillar type I, II, and III collagens.

板类型

Pre-coated

实验流程

rat MMP-8 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-8 has been precoated onto 96-well plates. Standards(NSO, L21-P466) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat MMP-8 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat MMP-8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat MMP-8 standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 486, Standard deviation: 19.93, CV(%): 4.1
- Sample 2: n=16, Mean(pg/ml): 1780, Standard deviation: 112.14, CV(%): 6.3
- Sample 3: n=16, Mean(pg/ml): 6826, Standard deviation: 354.95, CV(%): 5.2,
- Sample 1: n=24, Mean(pg/ml): 561, Standard deviation: 27.49, CV(%): 4.9
- Sample 2: n=24, Mean(pg/ml): 2175, Standard deviation: 158.78, CV(%): 7.3
- Sample 3: n=24, Mean(pg/ml): 7203, Standard deviation: 468.2, CV(%): 6.5
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Stio, Martinesi, Treves, Borgioli: "In vitro response of human peripheral blood mononuclear cells to AISI 316L austenitic stainless steel subjected to nitriding and collagen coating treatments." in: Journal of materials science. Materials in medicine, Vol. 26, Issue 2, pp. 100, (2015) (PubMed).
抗原 See all MMP8 ELISA试剂盒

MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

别名

MMP8

背景

Protein Function: Can degrade fibrillar type I, II, and III collagens.
Background: Matrix metalloproteinase 8(MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase(HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57 % identity with the deduced protein sequence for fibroblast collagenase with 72 % chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.
Synonyms: Neutrophil collagenase,3.4.24.34,Matrix metalloproteinase-8,MMP-8,Mmp8,
Full Gene Name: Neutrophil collagenase
Cellular Localisation: Cytoplasmic granule. Secreted, extracellular space, extracellular matrix . Stored in intracellular granules.

基因ID

63849

UniProt

O88766