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CXCL12 ELISA 试剂盒

Name: CXCL12 ELISA 试剂盒
SKU: ABIN2859208
Availability: OutOfStock

小鼠 CXCL12 ELISA Kit, Colorimetric is an assay for quantification of 小鼠 CXCL12, that has been mentioned in 4+ publications.

ELISA

1 image

(4 references)

产品编号 ABIN2859208

发货至: 中国

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Quick Overview for CXCL12 ELISA 试剂盒 (ABIN2859208)

抗原

See all CXCL12 ELISA试剂盒

CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

抗原表位

AA 22-93

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5 pg/mL - 4000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin)

Discover our top product CXCL12 ELISA Kit

最低检测浓度

62.5 pg/mL

原理

Sandwich Mouse SDF-1/CXCL12 ELISA Kit to quantitate Mouse CXCL12 in cell culture supernatants, serum and plasma (heparin).

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: K22-M93
Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

< 10 pg/mL

组件
1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers
试剂未包括

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) ELISA Kit
CXCL12 适用: 人 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 12 (CXCL12) ELISA Kit
CXCL12 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 12 (CXCL12) ELISA Kit
CXCL12 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 pg/mL - 50 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 12 (CXCL12) ELISA Kit
CXCL12 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 12 (CXCL12) ELISA Kit
CXCL12 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 12 (CXCL12) ELISA Kit
CXCL12 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 12 (CXCL12) ELISA Kit
CXCL12 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 12 (CXCL12) ELISA Kit
CXCL12 适用: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 12 (CXCL12) ELISA Kit
High Sensitivity CXCL12 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 12 (CXCL12) ELISA Kit
CXCL12 适用: 人 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

样本量

100 μL

板类型

Pre-coated

实验流程

ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

实验精密度

Intra-Assay Precision (CV%): 4.5%, 7.1%, 4.1%
Inter-Assay Precision (CV%): 4.9%, 5.2%, 5.8%

限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

有效期

12 months
Zhang, Teng, Liu, Zhang, Liu: "Gene expression profile analyze the molecular mechanism of CXCR7 regulating papillary thyroid carcinoma growth and metastasis." in: Journal of experimental & clinical cancer research : CR, Vol. 34, pp. 16, (2015) (PubMed).

Wu, Li, Wang, Shen, Xu, Liu, Zhuo, Xia, Gao, Tan: "Ultrasound-targeted stromal cell-derived factor-1-loaded microbubble destruction promotes mesenchymal stem cell homing to kidneys in diabetic nephropathy rats." in: International journal of nanomedicine, Vol. 9, pp. 5639-51, (2014) (PubMed).

Lin, Han, Wang, Xu, Yu, Yang: "CXCR7 stimulates MAPK signaling to regulate hepatocellular carcinoma progression." in: Cell death & disease, Vol. 5, pp. e1488, (2014) (PubMed).

Tong, Ding, Shen, Chen, Bian, Ma, Yao, Yang: "Mesenchymal stem cell transplantation enhancement in myocardial infarction rat model under ultrasound combined with nitric oxide microbubbles." in: PLoS ONE, Vol. 8, Issue 11, pp. e80186, (2013) (PubMed).
抗原 See all CXCL12 ELISA试剂盒

CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

别名

CXCL12

背景

Background: SDF-1 (stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12). This gene is located on chromosome 10q11.1. SDF-1 is produced in two forms, SDF-1alpha/CXCL12a and SDF-1beta/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes. CXCL12 was shown to be expressed in many tissues in mice (including brain, thymus, heart, lung, liver, kidney, spleen and bone marrow). CXCL12 is a highly efficacious lymphocyte chemoattractant. In addition, CXCL12 induces intracellular actin polymerization in lymphocytes. CXCL12 is a substrate for the matrix metalloproteinase-2, which cleaves an CXCL12 N-terminal tetrapeptide. The standard product used in this kit is recombinant SDF-1 with the molecular mass of 8Kda.
Gene Full Name: C-X-C motif chemokine ligand 12

基因ID

20315

UniProt

P40224

途径

Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling